Exploring protein interactions and intracellular localization in regulating flavonoid metabolism

Simple item page
dc.contributor.authorBowerman, Peter A.en
dc.contributor.committeechairWinkel, Brenda S. J.en
dc.contributor.committeememberGrabau, Elizabeth A.en
dc.contributor.committeememberSchubot, Florian D.en
dc.contributor.committeememberTholl, Dorotheaen
dc.contributor.departmentBiologyen
dc.date.accessioned2017-04-06T15:43:26Zen
dc.date.adate2010-09-14en
dc.date.available2017-04-06T15:43:26Zen
dc.date.issued2010-08-02en
dc.date.rdate2017-09-28en
dc.date.sdate2010-08-23en
dc.description.abstractThe organization of biological processes via protein-protein interactions and the subcellular localization of enzymes is believed to be fundamental to many aspects of metabolism. Although this organization has been demonstrated in several systems, the mechanisms by which it is established and regulated are still not well understood. The flavonoid biosynthetic pathway offers a unique system in which to study several important aspects of metabolism. Here we describe a novel toolset of mutant alleles within the flavonoid biosynthetic pathway. In addition, we discuss the use of several of these alleles together with a number of emerging technologies to probe the role of subcellular localization of chalcone synthase, the first committed flavonoid biosynthetic enzyme, on metabolic flux, and to characterize a novel chalcone synthase-interacting protein. The over-expression of this interacting protein induces novel phenotypes that are likely associated with the production or distribution of auxin. Further, interaction analyses between recombinant flavonoid biosynthetic enzymes point to the possibility that post-translational modifications play an important role in promoting interactions.en
dc.description.degreePh. D.en
dc.identifier.otheretd-08232010-153840en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08232010-153840/en
dc.identifier.urihttp://hdl.handle.net/10919/77174en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectprotein localizationen
dc.subjectflavonoiden
dc.subjectprotein-protein interactionsen
dc.titleExploring protein interactions and intracellular localization in regulating flavonoid metabolismen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
etd-08232010-153840_Bowerman_PA_D2010_version4.pdf
Size:
16.29 MB
Format:
Adobe Portable Document Format
Download

Collections

Doctoral Dissertations