Regulatory targets of quorum sensing in Vibrio cholerae: evidence for two distinct HapR-binding motifs

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dc.contributor.authorTsou, Amy M.en
dc.contributor.authorCai, Taoen
dc.contributor.authorLiu, Zhien
dc.contributor.authorZhu, Junen
dc.contributor.authorKulkarni, Rahul V.en
dc.contributor.departmentPhysicsen
dc.date.accessioned2019-04-18T14:46:56Zen
dc.date.available2019-04-18T14:46:56Zen
dc.date.issued2009-05en
dc.description.abstractThe quorum-sensing pathway in Vibrio cholerae controls the expression of the master regulator HapR, which in turn regulates several important processes such as virulence factor production and biofilm formation. While HapR is known to control several important phenotypes, there are only a few target genes known to be transcriptionally regulated by HapR. In this work, we combine bioinformatic analysis with experimental validation to discover a set of novel direct targets of HapR. Our results provide evidence for two distinct binding motifs for HapR-regulated genes in V. cholerae. The first binding motif is similar to the motifs recently discovered for orthologs of HapR in V. harveyi and V. vulnificus. However, our results demonstrate that this binding motif can be of variable length in V. cholerae. The second binding motif shares common elements with the first motif, but is of fixed length and lacks dyad symmetry at the ends. The contributions of different bases to HapR binding for this second motif were demonstrated using systematic mutagenesis experiments. The current analysis presents an approach for systematically expanding our knowledge of the quorum-sensing regulon in V. cholerae and other related bacteria.en
dc.description.notesThe Jeffress Foundation and a seed grant from the Institute for Critical Technology and Applied Science at Virginia Tech (to R. V. K); the National Institutes of Health (R01AI072479 to J.Z); a National Institutes of Health T32 Bacteriology training grant (to A. T); and an award from the China Scholarship Council (to T. C). Funding for open access charge: National Institutes of Health (R01AI072479).en
dc.description.sponsorshipInstitute for Critical Technology and Applied Science at Virginia Tech; National Institutes of Health [R01AI072479]; China Scholarship Councilen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/nar/gkp121en
dc.identifier.eissn1362-4962en
dc.identifier.issn0305-1048en
dc.identifier.issue8en
dc.identifier.pmid19276207en
dc.identifier.urihttp://hdl.handle.net/10919/89031en
dc.identifier.volume37en
dc.language.isoen_USen
dc.rightsCreative Commons Attribution-NonCommercial 2.0 Genericen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.0/en
dc.subjecto1 el-toren
dc.subjectvirulence gene-expressionen
dc.subjectcontrols biofilm formationen
dc.subjectsequence-analysis toolsen
dc.subjectvps biosynthesis genesen
dc.subjectnitric-oxideen
dc.subjectescherichia-colien
dc.subjectresponse regulatorsen
dc.subjectflavohemoglobin hmpen
dc.subjectapha promoteren
dc.titleRegulatory targets of quorum sensing in Vibrio cholerae: evidence for two distinct HapR-binding motifsen
dc.title.serialNucleic Acids Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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