Juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes

dc.contributor.authorLiu, Pengchengen
dc.contributor.authorFu, Xiaonanen
dc.contributor.authorZhu, Jinsongen
dc.contributor.departmentBiochemistryen
dc.date.accessioned2019-01-14T14:14:30Zen
dc.date.available2019-01-14T14:14:30Zen
dc.date.issued2018-08-14en
dc.date.updated2019-01-14T14:14:28Zen
dc.description.abstractJuvenile hormone (JH) regulates many aspects of insect development and reproduction. In some processes, JH plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20E). In Aedes aegypti mosquitoes, JH prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20E after blood ingestion. The molecular basis of this competence is still not well understood. Here, we report that JH regulates pre-mRNA splicing of the taiman gene, which encodes a key transcriptional regulator required for both JH- and 20E-controlled gene expression. JH stimulated the production of the Taiman isoforms A/B, while reducing the levels of the isoforms C/D, in the fat body after adult eclosion. The appearance of the A/B isoforms in maturing mosquitoes was accompanied by acquisition of the competence to respond to 20E. Depletion of the A/B isoforms, by inhibiting the alternative splicing or by isoform-specific RNA interference, considerably diminished the 20E-induced gene expression after a blood meal and substantially impaired oocyte development. In accordance with this observation, further studies indicated that in the presence of 20E, the Taiman A/B isoforms showed much stronger interactions with the 20E receptor complex than the Taiman C/D isoforms. In contrast, all four isoforms displayed similar capabilities of forming active JH receptor complexes with the methoprene-tolerant protein (Met). This study suggested that JH confers the competence to newly emerged female mosquitoes by regulating mRNA splicing to generate the Taiman isoforms that are essential for the vitellogenic 20E response.en
dc.description.versionPublished versionen
dc.format.extentPages E7738-E7747en
dc.format.extent10 page(s)en
dc.identifier.doihttps://doi.org/10.1073/pnas.1808146115en
dc.identifier.eissn1091-6490en
dc.identifier.issn0027-8424en
dc.identifier.issue33en
dc.identifier.orcidZhu, Jinsong [0000-0002-1587-753X]en
dc.identifier.other1808146115 (PII)en
dc.identifier.pmid30061397en
dc.identifier.urihttp://hdl.handle.net/10919/86674en
dc.identifier.volume115en
dc.language.isoenen
dc.publisherNational Academy of Sciencesen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000441638200013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectscience & technologyen
dc.subjectinsect hormoneen
dc.subjectrna splicingen
dc.subjectsignal transductionen
dc.subjectgene expressionen
dc.subjectcompetence factor beta-ftz-f1en
dc.subjectmethoprene-tolerant proteinen
dc.subjectaedes-aegypti mosquitosen
dc.subjecttranscriptional activationen
dc.subjectecdysone receptoren
dc.subjectexpressionen
dc.subjectcoactivatoren
dc.subject20-hydroxyecdysoneen
dc.subjectmetamorphosisen
dc.subjectepidermisen
dc.subjectrna splicingen
dc.subjectmd multidisciplinaryen
dc.subject.meshtesten
dc.subject.meshAnimalsen
dc.subject.meshAedesen
dc.subject.meshEcdysteroneen
dc.subject.meshJuvenile Hormonesen
dc.subject.meshInsect Proteinsen
dc.subject.meshProtein Isoformsen
dc.subject.meshTranscription Factorsen
dc.subject.meshFeeding Behavioren
dc.subject.meshVitellogenesisen
dc.subject.meshAlternative Splicingen
dc.subject.meshFemaleen
dc.titleJuvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoesen
dc.title.serialProceedings of The National Academy of Sciences of The United States of Americaen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
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