Growth Hormone-Regulated mRNAs and miRNAs in Chicken Hepatocytes

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dc.contributor.authorWang, Xingguoen
dc.contributor.authorYang, Leien
dc.contributor.authorWang, Huijuanen
dc.contributor.authorShao, Fangen
dc.contributor.authorYu, Jianfengen
dc.contributor.authorJiang, Honglinen
dc.contributor.authorHan, Yaopingen
dc.contributor.authorGong, Daoqingen
dc.contributor.authorGu, Zhiliangen
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2018-10-01T19:08:29Zen
dc.date.available2018-10-01T19:08:29Zen
dc.date.issued2014-11-11en
dc.description.abstractGrowth hormone (GH) is a key regulatory factor in animal growth, development and metabolism. Based on the expression level of the GH receptor, the chicken liver is a major target organ of GH, but the biological effects of GH on the chicken liver are not fully understood. In this work we identified mRNAs and miRNAs that are regulated by GH in primary hepatocytes from female chickens through RNA-seq, and analyzed the functional relevance of these mRNAs and miRNAs through GO enrichment analysis and miRNA target prediction. A total of 164 mRNAs were found to be differentially expressed between GH-treated and control chicken hepatocytes, of which 112 were up-regulated and 52 were down-regulated by GH. A total of 225 chicken miRNAs were identified by the RNA-Seq analysis. Among these miRNAs 16 were up-regulated and 1 miRNA was down-regulated by GH. The GH-regulated mRNAs were mainly involved in growth and metabolism. Most of the GH-upregulated or GH-downregulated miRNAs were predicted to target the GH-downregulated or GH-upregulated mRNAs, respectively, involved in lipid metabolism. This study reveals that GH regulates the expression of many mRNAs involved in metabolism in female chicken hepatocytes, which suggests that GH plays an important role in regulating liver metabolism in female chickens. The results of this study also support the hypothesis that GH regulates lipid metabolism in chicken liver in part by regulating the expression of miRNAs that target the mRNAs involved in lipid metabolism.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0112896en
dc.identifier.eissn1932-6203en
dc.identifier.issue11en
dc.identifier.othere112896en
dc.identifier.pmid25386791en
dc.identifier.urihttp://hdl.handle.net/10919/85205en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleGrowth Hormone-Regulated mRNAs and miRNAs in Chicken Hepatocytesen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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