Role of autocrine growth factors in the tumorigenic transformation of T cells

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dc.contributor.authorHassuneh, Mona Rushdien
dc.contributor.committeecochairNagarkatti, Mitzien
dc.contributor.committeecochairNagarkatti, Prakash S.en
dc.contributor.committeememberSchurig, Gerhardt G.en
dc.contributor.committeememberTyson, John J.en
dc.contributor.committeememberWong, Eric A.en
dc.contributor.departmentBiologyen
dc.date.accessioned2014-03-14T21:12:34Zen
dc.date.adate2008-06-06en
dc.date.available2014-03-14T21:12:34Zen
dc.date.issued1996-04-05en
dc.date.rdate2008-06-06en
dc.date.sdate2008-06-06en
dc.description.abstractIn the current study we tested the hypothesis that tumorigenic transformation of T cells may result from aberrant regulation of autocrine growth factor production. We describe for the first time characterization of several normal T cell lines that underwent spontaneous transformation resulting from constitutive expression of interleukin-2 (IL-2) and interleukin 2 receptor (IL-2R) genes. Such cell lines could induce tumors <i>in vivo</i> and furthermore, the growth of such transformed cells both <i>ex vivo</i> and <i>in vivo</i> could be inhibited by monoclonal antibodies (mAbs) or antisense oligonucleotides specific for IL-2 or IL-2R. Interestingly, an <i>in vivo</i> originated T cell lymphoma, LSA, was also found to be dependent on constitutive production of IL-2 and Interleul(in-4 (IL-4). Together, these data demonstrated that dysregulation in the production or responsiveness to autocrine T cell growth factors, plays an important role in T cell transformation and tumorigenicity. Due to their ability to produce T cell growth factors, such tumor cells were found to be highly immunogenic. Thus it was surprising that some T cell lymphomas could still grow in an immunocompetent host and kill the host. To this effect, we investigated additional mechanisms used by such tumor cell lines to grow in an immunocompetent host. It was noted that the tumor cells used mechanisms such as failure to express MHC and production of immunosuppressive cytokines, such as, transforming growth factor-β (TGF-β) and interleukin-10 (IL-10), to evade the action of the immune system. In addition, the T cell lymphomas constitutively expressed F as-ligand and triggered apoptosis of host T cells that expressed Fas. Together, the current study suggests that cytokines produced by the tumor cells play an important role in tumorigenic transformation as vvell as maintenance of tumor growth in an immunocompetent host.en
dc.description.degreePh. D.en
dc.format.extentxviii, 166 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-06062008-154644en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06062008-154644/en
dc.identifier.urihttp://hdl.handle.net/10919/38085en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1996.H377.pdfen
dc.relation.isformatofOCLC# 35109964en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjecttumor cellsen
dc.subject.lccLD5655.V856 1996.H377en
dc.titleRole of autocrine growth factors in the tumorigenic transformation of T cellsen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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