An evaluation of a new rapid qPCR test for the detection of 2019-novel coronavirus nucleocapsid (N1) gene in wastewater in Roanoke and Salem VA sewersheds

dc.contributor.authorLehrer, Lia W.en
dc.contributor.authorLewis, Annaen
dc.contributor.authorTolliver, Susan A.en
dc.contributor.authorDegen, Marciaen
dc.contributor.authorSingh, Rekhaen
dc.contributor.authorHouser, Sara R.en
dc.contributor.authorRao, Jayasimhaen
dc.date.accessioned2024-11-07T20:36:37Zen
dc.date.available2024-11-07T20:36:37Zen
dc.date.issued2024-08en
dc.description.abstractThe COVID-19 pandemic initiated public interest in wastewater-based epidemiology (WBE). Public and private entities responded to the need to produce timely and accurate data. LuminUltra and Hach partnered to provide a rapid, field-based quantitative polymerase chain reaction (qPCR) test for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in wastewater. This study evaluates the Hach GeneCount SARS-CoV-2 Wastewater RT-qPCR Assay Kit and LuminUltra GeneCount® Q-16 RT-PCR instrument. The Hach LuminUltra methods were compared to the Promega Wizard® Enviro Total Nucleic Acid kit and Bio-Rad CFX Opus 96 Real-time PCR Detection System. Over a 12-week period, wastewater samples were collected weekly from seven locations in the Roanoke/Salem, VA sewersheds. Concentration and extraction of the viral RNA were followed by qPCR analysis. The target gene for detection was the nucleocapsid gene (N1) of the SARS-CoV-2 virus. Costs, ease of use, time to produce results, sample preparation, and data comparisons were considered. The comparison determined that the Hach LuminUltra method and instrument were more affordable, consumed less time, and required less technical expertise. While the new method was specific, it had low sensitivity. This evaluation suggests the Hach LuminUltra method should be reserved for limited situations requiring onsite field analysis where data accuracy is not essential.en
dc.description.versionPublished versionen
dc.format.extentPages 1419-1428en
dc.format.extent10 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.2166/wh.2024.085en
dc.identifier.eissn1996-7829en
dc.identifier.issn1477-8920en
dc.identifier.issue8en
dc.identifier.orcidRao, Jayasimha [0000-0002-0133-2862]en
dc.identifier.pmid39212279en
dc.identifier.urihttps://hdl.handle.net/10919/121578en
dc.identifier.volume22en
dc.language.isoenen
dc.publisherIWA Publishingen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/39212279en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCOVID-19en
dc.subjectN1 geneen
dc.subjectqPCRen
dc.subjectSouthwest Virginiaen
dc.subjectwastewater-based epidemiologyen
dc.subjectwastewater surveillanceen
dc.subject.meshHumansen
dc.subject.meshPhosphoproteinsen
dc.subject.meshRNA, Viralen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshSewageen
dc.subject.meshReal-Time Polymerase Chain Reactionen
dc.subject.meshCOVID-19en
dc.subject.meshSARS-CoV-2en
dc.subject.meshCoronavirus Nucleocapsid Proteinsen
dc.subject.meshWastewateren
dc.titleAn evaluation of a new rapid qPCR test for the detection of 2019-novel coronavirus nucleocapsid (N1) gene in wastewater in Roanoke and Salem VA sewershedsen
dc.title.serialJournal of Water and Healthen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2024-07-20en
pubs.finish-date2023-04-04en
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Faculty of Health Sciencesen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicine/General IMen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/TEACH Membersen
pubs.start-date2023-04-04en

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