Effects of Dairy Manure-Based Amendments and Soil Texture on Lettuce-and Radish-Associated Microbiota and Resistomes

dc.contributor.authorGuron, Giselle K.P.en
dc.contributor.authorArango-Argoty, Gustavoen
dc.contributor.authorZhang, Liqingen
dc.contributor.authorPruden, Amyen
dc.contributor.authorPonder, Monica A.en
dc.contributor.departmentCivil and Environmental Engineeringen
dc.description.abstractDairy cattle are routinely treated with antibiotics, and the resulting manure or composted manure is commonly used as a soil amendment for crop production, raising questions regarding the potential for antibiotic resistance to propagate from “farm to fork.” The objective of this study was to compare the microbiota and “resistomes” (i.e., carriage of antibiotic resistance genes [ARGs]) associated with lettuce leaf and radish taproot surfaces grown in different soils amended with dairy manure, compost, or chemical fertilizer only (control). Manure was collected from antibiotic-free dairy cattle (DC) or antibiotic-treated dairy cattle (DA), with a portion composted for parallel comparison. Amendments were applied to loamy sand or silty clay loam, and lettuce and radishes were cultivated to maturity in a greenhouse. Metagenomes were profiled via shotgun Illumina sequencing. Radishes carried a distinct ARG composition compared to that of lettuce, with greater relative abundance of total ARGs. Taxonomic species richness was also greater for radishes by 1.5-fold. The resistomes of lettuce grown with DC compost were distinct from those grown with DA compost, DC manure, or fertilizer only. Further, compost applied to loamy sand resulted in twofold-greater relative abundance of total ARGs on lettuce than when applied to silty clay loam. The resistomes of radishes grown with biological amendments were distinct from the corresponding fertilizer controls, but effects of composting or antibiotic use were not measureable. Cultivation in loamy sand resulted in higher species richness for both lettuce and radishes than when grown in silty clay loam by 2.2-fold and 1.2-fold, respectively, when amended with compost.en
dc.description.sponsorshipFunding was supported by USDA NIFA-AFRI 2014-05280/2015-68003-23050 and 2017-68003-26498. In addition, A.P. was supported by the NSF NNCI grant 1542100, VT ICTAS Center for Science and Engineering of the Exposome, and M.A.P. was supported in part by the Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture. The VT Open Access Subvention Fund contributed to publishing fees for this article.en
dc.format.extent14 pagesen
dc.identifier.citationGuron GKP, Arango-Argoty G, Zhang L, Pruden A, Ponder MA. 2019. Effects of dairy manure-based amendments and soil texture on lettuce- and radish-associated microbiota and resistomes. mSphere 4:e00239-19. https:// doi.org/10.1128/mSphere.00239-19.en
dc.publisherAmerican Society for Microbiologyen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.subjectantibiotic resistanceen
dc.subjectvegetable microbiomeen
dc.titleEffects of Dairy Manure-Based Amendments and Soil Texture on Lettuce-and Radish-Associated Microbiota and Resistomesen
dc.typeArticle - Refereeden


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