Development and ultrastructure of cystoisospora canis nemeseri, 1959 (syn. isospora canis) monozoic cysts in two noncanine cell lines

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dc.contributor.authorMitchell, S. M.en
dc.contributor.authorZajac, Anne M.en
dc.contributor.authorLindsay, David S.en
dc.date.accessed2014-06-16en
dc.date.accessioned2014-06-20T14:13:11Zen
dc.date.available2014-06-20T14:13:11Zen
dc.date.issued2009-08en
dc.description.abstractCystoisospora canis is a coccidial parasite of the intestinal tract that can cause severe disease in dogs. Clinical Signs include watery diarrhea, vomiting, fever, and weight loss. Extraintestinal stages of Cystoisospora spp. have been demonstrated in the mesenteric lymph nodes of paratenic hosts. information on the biology of extraintestinal stages of canine Cystoisospora species is limited. The Current study examined the development of C canis in 2 noncanine cell lines and the ultrastructure of the monozoic cysts that formed. Monolayers of bovine turbinate cells and African green monkey kidney cells were grown on coverslips and inoculated with excysted C canis sporozoites. Coverslips were collected oil various days and fixed and stained for light microscopy (LM) or transmission electron microscopy (TEM). A single, centrally located, slightly crescent-shaped sporozoite Surrounded by a thick cyst wall within a parasitophorous vacuole was observed with the use of LM and TEM. No division and no multinucleated stages were observed with either LM or TEM. With TEM, typical organelles of sporozoites were observed, such as rhoptries, dense granules, a crystalloid body, polysaccharide granules, and a conoid. The structure and ultrastructure of C. canis monozoic cysts produced in vitro are similar to extraintestinal cysts of other Cystoisospora species in experimentally infected animals and those of Cystoisospora belli observed in immunocompromised humans. This is the first Study that fully demonstrates in vitro the development of what structurally resemble extraintestinal cysts or a Cystoisospora spp.en
dc.description.sponsorshipBayer HealthCare Animal Healthen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSheila M. Mitchell, Anne M. Zajac, and David S. Lindsay (2009). "Development and Ultrastructure of Cystoisospora canis Nemeseri, 1959 (syn. Isospora canis) Monozoic Cysts in Two Noncanine Cell Lines," Journal of Parasitology, Vol. 95, No. 4, pp. 793-798. doi: http://dx.doi.org/10.1645/GE-1951.1en
dc.identifier.doihttps://doi.org/10.1645/ge-1951.1en
dc.identifier.issn0022-3395en
dc.identifier.urihttp://hdl.handle.net/10919/49036en
dc.identifier.urlhttp://www.bioone.org/doi/abs/10.1645/GE-1951.1en
dc.language.isoenen
dc.publisherAmerican Society of Parasitologyen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectimmune-deficiency-syndromeen
dc.subjectcultured-cellsen
dc.subjectlife-cycleen
dc.subjectcytologicalen
dc.subjectobservationsen
dc.subjecttissue cystsen
dc.subjectdogsen
dc.subjectsuisen
dc.subjectrivoltaen
dc.subjectpatienten
dc.subjectfelisen
dc.subjectparasitologyen
dc.titleDevelopment and ultrastructure of cystoisospora canis nemeseri, 1959 (syn. isospora canis) monozoic cysts in two noncanine cell linesen
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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