The characterization of Clostridium beijerinckii NRRL B592 cells transformed with plasmids containing the butanol-production genes under the control of constitutive promoters

Simple item page
dc.contributor.authorTollin, Craig Jeffreyen
dc.contributor.committeechairChen, Jiann-Shinen
dc.contributor.committeememberLarson, Timothy J.en
dc.contributor.committeememberBevan, David R.en
dc.contributor.committeememberMelville, Stephen B.en
dc.contributor.departmentBiochemistryen
dc.date.accessioned2017-04-06T15:44:01Zen
dc.date.adate2012-12-07en
dc.date.available2017-04-06T15:44:01Zen
dc.date.issued2012-09-18en
dc.date.rdate2016-10-18en
dc.date.sdate2012-10-02en
dc.description.abstractClostridium beijerinckii is a spore-forming, obligate anaerobe that is capable of producing butanol, acetone and isopropanol. These industrial chemicals are traditionally known as solvents. The regulation of solventogenic fermentation is linked to the onset of sporulation, so that by the time the organism begins to produce solvents, it is also entering into spore formation and metabolic slowdown. The goal of this research project was to study the effect of placing the solvent-production genes from C. beijerinckii under the control of constitutive promoters from other genes, in an attempt to allow an earlier start of butanol production during the growth phase than is the case with the wild-type cells. The aldehyde dehydrogenase from C. beijerinckii NRRL B593 (ald) and alcohol dehydrogenase from C. beijerinckii NRRL B592 (adhA) were placed under the control of the promoter from the acid-producing operon (the BCS operon) in one vector, and under the control of the promoter from the ferredoxin gene in another. In both cases, aldehyde dehydrogenase activity was produced earlier in the growth phase in transformed cells, but alcohol dehydrogenase activity was not. The adhA gene from C. beijerinckii NRRL B592 was paired with the adhB gene from the same organism in a third vector, both under the control of the promoter from the BCS operon. In cells transformed with this vector, alcohol dehydrogenase activity was observed earlier in the growth phase than it was in wild-type NRRL B592 cells.en
dc.description.degreePh. D.en
dc.identifier.otheretd-10022012-143748en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10022012-143748/en
dc.identifier.urihttp://hdl.handle.net/10919/77235en
dc.language.isoen_USen
dc.publisherVirginia Techen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectferredoxinen
dc.subjectbcsen
dc.subjectClostridiumen
dc.subjectsolventogenesisen
dc.subjectadhAen
dc.subjectalden
dc.subjectanaerobeen
dc.titleThe characterization of Clostridium beijerinckii NRRL B592 cells transformed with plasmids containing the butanol-production genes under the control of constitutive promotersen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
etd-10022012-143748_Tollin_CT_D_2012.pdf
Size:
2.66 MB
Format:
Adobe Portable Document Format
Download

Collections

Doctoral Dissertations