Genetic and morphological characterization of the freshwater mussel clubshell species complex (Pleurobema clava and Pleurobema oviforme) to inform conservation planning

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dc.contributor.authorMorrison, Cheryl L.en
dc.contributor.authorJohnson, Nathan A.en
dc.contributor.authorJones, Jess W.en
dc.contributor.authorEackles, Michael S.en
dc.contributor.authorAunins, Aaron W.en
dc.contributor.authorFitzgerald, Daniel B.en
dc.contributor.authorHallerman, Eric M.en
dc.contributor.authorKing, Tim L.en
dc.date.accessioned2022-02-06T16:55:36Zen
dc.date.available2022-02-06T16:55:36Zen
dc.date.issued2021-10-20en
dc.date.updated2022-02-06T16:55:33Zen
dc.description.abstractThe shell morphologies of the freshwater mussel species Pleurobema clava (federally endangered) and Pleurobema oviforme (species of concern) are similar, causing considerable taxonomic confusion between the two species over the last 100 years. While P. clava was historically widespread throughout the Ohio River basin and tributaries to the lower Laurentian Great Lakes, P. oviforme was confined to the Tennessee and the upper Cumberland River basins. We used two mitochondrial DNA (mtDNA) genes, 13 novel nuclear DNA microsatellite markers, and shell morphometrics to help resolve this taxonomic confusion. Evidence for a single species was apparent in phylogenetic analyses of each mtDNA gene, revealing monophyletic relationships with minimal differentiation and shared haplotypes. Analyses of microsatellites showed significant genetic structuring, with four main genetic clusters detected, respectively, in the upper Ohio River basin, the lower Ohio River and Great Lakes, and upper Tennessee River basin, and a fourth genetic cluster, which included geographically intermediate populations in the Ohio and Tennessee river basins. While principal components analysis (PCA) of morphometric variables (i.e., length, height, width, and weight) showed significant differences in shell shape, only 3% of the variance in shell shape was explained by nominal species. Using Linear Discriminant and Random Forest (RF) analyses, correct classification rates for the two species' shell forms were 65.5% and 83.2%, respectively. Random Forest classification rates for some populations were higher; for example, for North Fork Holston (HOLS), it was >90%. While nuclear DNA and shell morphology indicate that the HOLS population is strongly differentiated, perhaps indicative of cryptic biodiversity, we consider the presence of a single widespread species the most likely biological scenario for many of the investigated populations based on our mtDNA dataset. However, additional sampling of P. oviforme populations at nuclear loci is needed to corroborate this finding.en
dc.description.versionPublished versionen
dc.format.extentPages 15325-15350en
dc.format.extent26 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1002/ece3.8219en
dc.identifier.eissn2045-7758en
dc.identifier.issn2045-7758en
dc.identifier.issue21en
dc.identifier.orcidHallerman, Eric [0000-0002-5839-858X]en
dc.identifier.otherECE38219 (PII)en
dc.identifier.pmid34765181en
dc.identifier.urihttp://hdl.handle.net/10919/108155en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000709198500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectEcologyen
dc.subjectEvolutionary Biologyen
dc.subjectEnvironmental Sciences & Ecologyen
dc.subjectCOIen
dc.subjectcryptic biodiversityen
dc.subjectendangered speciesen
dc.subjectmicrosatellite DNAen
dc.subjectmitochondrial DNAen
dc.subjectmorphometricsen
dc.subjectNDIen
dc.subjectNORTHERN RIFFLESHELL MUSSELen
dc.subjectTORULOSA-RANGIANA BIVALVIAen
dc.subjectPOPULATION-STRUCTUREen
dc.subjectMITOCHONDRIAL-DNAen
dc.subjectINTRASPECIFIC PHYLOGEOGRAPHYen
dc.subjectMOLECULAR SYSTEMATICSen
dc.subjectCOMPUTER-PROGRAMen
dc.subjectFUSCONAIA-FLAVAen
dc.subjectWABASH PIGTOEen
dc.subjectUNIONIDAEen
dc.subjectCOIen
dc.subjectNDIen
dc.subject0602 Ecologyen
dc.subject0603 Evolutionary Biologyen
dc.titleGenetic and morphological characterization of the freshwater mussel clubshell species complex (Pleurobema clava and Pleurobema oviforme) to inform conservation planningen
dc.title.serialEcology and Evolutionen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2021-08-26en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Natural Resources & Environmenten
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/Fish and Wildlife Conservationen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Natural Resources & Environment/CNRE T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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