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Biodegradation of pesticide and indolic compounds under methanogenic conditions

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dc.contributor.authorGu, Ji-Dongen
dc.contributor.committeechairBerry, Duane F.en
dc.contributor.committeecochairMartens, David C.en
dc.contributor.committeememberEdmonds, William J.en
dc.contributor.committeememberRobinson, Joseph A.en
dc.contributor.committeememberZelazny, Lucian W.en
dc.contributor.departmentCrop and Soil Environmental Sciencesen
dc.date.accessioned2014-03-14T21:21:07Zen
dc.date.adate2005-10-13en
dc.date.available2014-03-14T21:21:07Zen
dc.date.issued1991-10-15en
dc.date.rdate2005-10-13en
dc.date.sdate2005-10-13en
dc.description.abstractDegradability of atrazine, cyanazine, and dicamba under methanogenic conditions was evaluated using serum bottle microcosms containing wetland soil inocula obtained from three different sites. Pesticides were monitored by high-performance liquid chromatography (HPLC) and the production of methane was measured with a gas chromatograph (GC). Dicamba was the most susceptible to degradation in the microcosms, followed by cyanazine. Atrazine was not degraded in the wetland soils. A dicamba-degrading methanogenic consortium was enriched from one of the initial wetland soil microcosms (Lawnes). Dicamba degradation was further examined using this consortium. Net methane production suggested that the aromatic ring was not degraded. Rates of dicamba degradation were enhanced with addition of 0.2 % yeast extract. Dicamba degradation was accomplished within 4 days compared to 22 days without yeast extract addition. The inability of the consortium to degrade the benzenoid ring was confirmed when no ¹⁴CO₂ was produced upon addition of [U-¹⁴C]dicamba to the cultures. Analysis of culture filtrate by HPLC revealed the presence of a possible metabolite that was aromatic in character.en
dc.description.degreePh. D.en
dc.format.extentxix, 145 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10132005-152539en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10132005-152539/en
dc.identifier.urihttp://hdl.handle.net/10919/39831en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1991.G8.pdfen
dc.relation.isformatofOCLC# 25140653en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1991.G8en
dc.subject.lcshBiodegradationen
dc.subject.lcshHeterocyclic compoundsen
dc.subject.lcshIndole alkaloidsen
dc.subject.lcshPesticidesen
dc.titleBiodegradation of pesticide and indolic compounds under methanogenic conditionsen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplineCrop and Soil Environmental Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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