Sarcocystis Strixi N. Sp From a Barred Owl (Strix varia) Definitive Host and Interferon Gamma Gene Knockout Mice as Experimental Intermediate Host

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dc.contributor.authorVerma, S. K.en
dc.contributor.authorvon Dohlen, Alexa Rosypalen
dc.contributor.authorMowery, J. D.en
dc.contributor.authorScott, D.en
dc.contributor.authorCerqueira-Cezar, Camila K.en
dc.contributor.authorRosenthal, B. M.en
dc.contributor.authorDubey, Jitender P.en
dc.contributor.authorLindsay, David S.en
dc.date.accessioned2020-03-17T17:26:46Zen
dc.date.available2020-03-17T17:26:46Zen
dc.date.issued2017-12en
dc.description.abstractHere we report a new species of Sarcocystis with a barred owl (Strix varia) as the natural definitive host and interferon gamma gene knockout (KO) mice as an experimental intermediate host. A barred owl submitted to the Carolina Raptor Center, Huntersville, North Carolina, was euthanized because of paralysis. Fully sporulated 12.539.9 lm sporocysts were found in intestinal scrapings from the owl. Sporocysts from the barred owl were orally fed to 4 laboratory-reared outbred Swiss Webster (SW) (Mus musculus) and 8 KO mice. All mice remained asymptomatic. Microscopic sarcocysts were found in all 5 KO mice euthanized on day 32, 59, 120, 154, and 206 post- inoculation (PI), not in KO mice euthanized on day 4, 8, and 14 PI. Sarcocysts were not found in any SW mice euthanized on day 72, 120, 206, and 210 PI. Sarcocysts were microscopic, up to 70 lm wide. By light microscopy, the sarcocyst wall, 2 lm thick had undulating, flat to conical, protrusions of varying dimensions. Numerous sarcocysts were seen in the histological sections of tongue and skeletal muscles from the abdomen, limbs, and eye but not in the heart. By transmission electron microscopy, the sarcocyst wall was "type 1j.'' The ground substance layer (gs) was homogenous, up to 2 lm thick, with very fine granules, and a few vesicles concentrated toward the villar projections. No microtubules were seen in the gs. Longitudinally cut bradyzoites at 206 days PI were 7.8 3 2.2 lm. Based on molecular characterization using 18S rRNA, 28S rRNA, and cox1 genes and morphology of sarcocysts, the parasite in the present study was biologically and structurally different from species so far described, and we therefore propose a new species name, Sarcocystis strixi n. sp.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.notesThis work was supported by grant 1505407 from the National Science Foundation Historically Black Colleges and Universities Undergraduate Program to A.R. von D. and an IRC grant from the Virginia-Maryland College of Veterinary Medicine to D.S.L. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA; USDA is an equal opportunity provider and employer.en
dc.description.sponsorshipNational Science Foundation Historically Black Colleges and Universities Undergraduate Program [1505407]; IRC grant from the Virginia-Maryland College of Veterinary Medicineen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1645/16-173en
dc.identifier.eissn1937-2345en
dc.identifier.issn0022-3395en
dc.identifier.issue6en
dc.identifier.pmid28783438en
dc.identifier.urihttp://hdl.handle.net/10919/97335en
dc.identifier.volume103en
dc.language.isoenen
dc.rightsCreative Commons CC0 1.0 Universal Public Domain Dedicationen
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/en
dc.titleSarcocystis Strixi N. Sp From a Barred Owl (Strix varia) Definitive Host and Interferon Gamma Gene Knockout Mice as Experimental Intermediate Hosten
dc.title.serialJournal of Parasitologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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