Flow Measurements in a Blood-Perfused Collagen Vessel Using X-Ray Micro-Particle Image Velocimetry

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dc.contributorVirginia Tech. Department of Mechanical Engineeringen
dc.contributorVirginia Tech-Wake Forest University School of Biomedical Engineering and Sciencesen
dc.contributor.authorAntoine, Elizabeth E.en
dc.contributor.authorBuchanan, Caraen
dc.contributor.authorFezzaa, Kamelen
dc.contributor.authorLee, Wah-Keaten
dc.contributor.authorRylander, M. Nicholeen
dc.contributor.authorVlachos, Pavlos P.en
dc.contributor.departmentMechanical Engineeringen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2015-10-01T23:25:30Zen
dc.date.available2015-10-01T23:25:30Zen
dc.date.issued2013-11-18en
dc.description.abstractBlood-perfused tissue models are joining the emerging field of tumor engineering because they provide new avenues for modulation of the tumor microenvironment and preclinical evaluation of the therapeutic potential of new treatments. The characterization of fluid flow parameters in such in-vitro perfused tissue models is a critical step towards better understanding and manipulating the tumor microenvironment. However, traditional optical flow measurement methods are inapplicable because of the opacity of blood and the thickness of the tissue sample. In order to overcome the limitations of optical method we demonstrate the feasibility of using phase-contrast x-ray imaging to perform microscale particle image velocimetry (PIV) measurements of flow in blood perfused hydrated tissue-representative microvessels. However, phase contrast x-ray images significantly depart from the traditional PIV image paradigm, as they have high intensity background, very low signal-to-noise ratio, and volume integration effects. Hence, in order to achieve accurate measurements special attention must be paid to the image processing and PIV cross-correlation methodologies. Therefore we develop and demonstrate a methodology that incorporates image preprocessing as well as advanced PIV cross-correlation methods to result in measured velocities within experimental uncertainty.en
dc.description.sponsorshipHenry Luce Foundationen
dc.description.sponsorshipVirginia Polytechnic Institute and State Universityen
dc.description.sponsorshipVirginia Space Grant Consortiumen
dc.description.sponsorshipVirginia Polytechnic Institute and State University. Institute of Critical Technology and Applied Sciencesen
dc.description.sponsorshipU.S. Department of Energyen
dc.description.sponsorshipBasic Energy Sciencesen
dc.description.sponsorshipClare Booth Luce Graduate Fellowshipen
dc.description.sponsorshipVirginia Space Grant Consortium Graduate STEM Research Fellowshipen
dc.format.extent8 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAntoine, E., Buchanan, C., Fezzaa, K., Lee, W.-K., Rylander, M. N., & Vlachos, P. (2013). Flow Measurements in a Blood-Perfused Collagen Vessel Using X-Ray Micro-Particle Image Velocimetry. Plos One, 8(11), e81198. doi: 10.1371/journal.pone.0081198en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0081198en
dc.identifier.issn1932-6203en
dc.identifier.issue11en
dc.identifier.urihttp://hdl.handle.net/10919/56684en
dc.identifier.volume8en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectCanceren
dc.subjectCellen
dc.subjectIn-vitroen
dc.subjectMicro-PIV measurementsen
dc.subjectMicrofluidic culture modelsen
dc.subjectShear stressen
dc.subjectSolid tumorsen
dc.subjectSquare microchannelen
dc.subjectTumor angiogenesisen
dc.subjectVelocity fieldsen
dc.titleFlow Measurements in a Blood-Perfused Collagen Vessel Using X-Ray Micro-Particle Image Velocimetryen
dc.title.serialPloS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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