Cancer detection in dogs using rapid Raman molecular urinalysis

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dc.contributor.authorRobertson, John L.en
dc.contributor.authorDervisis, Nikolaos G.en
dc.contributor.authorRossmeisl, John H. Jr.en
dc.contributor.authorNightengale, Marlieen
dc.contributor.authorFields, Danielen
dc.contributor.authorDedrick, Cameronen
dc.contributor.authorNgo, Laceyen
dc.contributor.authorIssa, Amr Sayeden
dc.contributor.authorGuruli, Georgien
dc.contributor.authorOrlando, Giuseppeen
dc.contributor.authorSenger, Ryan S.en
dc.date.accessioned2025-01-23T14:14:30Zen
dc.date.available2025-01-23T14:14:30Zen
dc.date.issued2024-02-07en
dc.description.abstractIntroduction: The presence of cancer in dogs was detected by Raman spectroscopy of urine samples and chemometric analysis of spectroscopic data. The procedure created a multimolecular spectral fingerprint with hundreds of features related directly to the chemical composition of the urine specimen. These were then used to detect the broad presence of cancer in dog urine as well as the specific presence of lymphoma, urothelial carcinoma, osteosarcoma, and mast cell tumor. Methods: Urine samples were collected via voiding, cystocentesis, or catheterization from 89 dogs with no history or evidence of neoplastic disease, 100 dogs diagnosed with cancer, and 16 dogs diagnosed with non-neoplastic urinary tract or renal disease. Raman spectra were obtained of the unprocessed bulk liquid urine samples and were analyzed by ISREA, principal component analysis (PCA), and discriminant analysis of principal components (DAPC) were applied using the Rametrix®Toolbox software. Results and discussion: The procedure identified a spectral fingerprint for cancer in canine urine, resulting in a urine screening test with 92.7% overall accuracy for a cancer vs. cancer-free designation. The urine screen performed with 94.0% sensitivity, 90.5% specificity, 94.5% positive predictive value (PPV), 89.6% negative predictive value (NPV), 9.9 positive likelihood ratio (LR+), and 0.067 negative likelihood ratio (LR-). Raman bands responsible for discerning cancer were extracted from the analysis and biomolecular associations were obtained. The urine screen was more effective in distinguishing urothelial carcinoma from the other cancers mentioned above. Detection and classification of cancer in dogs using a simple, non-invasive, rapid urine screen (as compared to liquid biopsies using peripheral blood samples) is a critical advancement in case management and treatment, especially in breeds predisposed to specific types of cancer.en
dc.description.versionPublished versionen
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 1328058 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fvets.2024.1328058en
dc.identifier.eissn2297-1769en
dc.identifier.issn2297-1769en
dc.identifier.orcidDervisis, Nikolaos [0000-0003-2869-1483]en
dc.identifier.orcidRobertson, John [0000-0002-5499-9943]en
dc.identifier.orcidSenger, Ryan [0000-0002-2450-6693]en
dc.identifier.otherPMC10879274en
dc.identifier.pmid38384948en
dc.identifier.urihttps://hdl.handle.net/10919/124322en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/38384948en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectRaman spectroscopyen
dc.subjectcanceren
dc.subjectlymphomaen
dc.subjecturineen
dc.subjectchemometricen
dc.subjectosteosarcomaen
dc.subjectmast cell tumoren
dc.subjecturothelial carcinoma (UC)en
dc.titleCancer detection in dogs using rapid Raman molecular urinalysisen
dc.title.serialFrontiers in Veterinary Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2024-01-18en
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciencesen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/Biological Systems Engineeringen
pubs.organisational-groupVirginia Tech/Engineeringen
pubs.organisational-groupVirginia Tech/Veterinary Medicineen
pubs.organisational-groupVirginia Tech/Veterinary Medicine/Small Animal Clinical Sciencesen
pubs.organisational-groupVirginia Tech/Engineering/Biomedical Engineering and Mechanicsen
pubs.organisational-groupVirginia Tech/Faculty of Health Sciencesen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicine/Secondary Appointment- Internal Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicine/Internal Med-Subgroupen

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