Overexpression of AtLOV1 in Switchgrass Alters Plant Architecture, Lignin Content, and Flowering Time

dc.contributor.authorXu, Binen
dc.contributor.authorSathitsuksanoh, Noppadonen
dc.contributor.authorTang, Yuhongen
dc.contributor.authorUdvardi, Michael K.en
dc.contributor.authorZhang, Ji-Yien
dc.contributor.authorShen, Zhengxingen
dc.contributor.authorBalota, Mariaen
dc.contributor.authorHarich, Kimen
dc.contributor.authorZhang, Y. H. Percivalen
dc.contributor.authorZhao, Bingyu Y.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentBiological Systems Engineeringen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2015-10-01T23:24:47Zen
dc.date.available2015-10-01T23:24:47Zen
dc.date.issued2012-12-26en
dc.description.abstractBackground: Switchgrass (Panicum virgatum L.) is a prime candidate crop for biofuel feedstock production in the United States. As it is a self-incompatible polyploid perennial species, breeding elite and stable switchgrass cultivars with traditional breeding methods is very challenging. Translational genomics may contribute significantly to the genetic improvement of switchgrass, especially for the incorporation of elite traits that are absent in natural switchgrass populations. Methodology/Principal Findings: In this study, we constitutively expressed an Arabidopsis NAC transcriptional factor gene, LONG VEGETATIVE PHASE ONE (AtLOV1), in switchgrass. Overexpression of AtLOV1 in switchgrass caused the plants to have a smaller leaf angle by changing the morphology and organization of epidermal cells in the leaf collar region. Also, overexpression of AtLOV1 altered the lignin content and the monolignol composition of cell walls, and caused delayed flowering time. Global gene-expression analysis of the transgenic plants revealed an array of responding genes with predicted functions in plant development, cell wall biosynthesis, and flowering. Conclusions/Significance: To our knowledge, this is the first report of a single ectopically expressed transcription factor altering the leaf angle, cell wall composition, and flowering time of switchgrass, therefore demonstrating the potential advantage of translational genomics for the genetic improvement of this crop.en
dc.description.sponsorshipU.S. Department of Energyen
dc.description.sponsorshipVirginia Polytechnic and State Universityen
dc.description.sponsorshipVirginia Polytechnic Institute and State University. Institute for Critical Technology and Applied Scienceen
dc.description.sponsorshipVirginia Polytechnic Institute and State University. College of Agriculture and Life Sciences. Biodesign and Bioprocessing Research Centeren
dc.description.sponsorshipVirginia Agricultural Experimental Stationen
dc.description.sponsorshipU.S. Department of Energyen
dc.description.sponsorshipBioEnergy Science Center (U.S.)en
dc.description.sponsorshipDE-FG02-08ER64629en
dc.description.sponsorshipVA135872en
dc.format.extent11 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationXu, B., Sathitsuksanoh, N., Tang, Y., Udvardi, M. K., Zhang, J.-Y., Shen, Z., Balota, M., Harich, K., Zhang, P. Y. H., & Zhao, B. (2012). Overexpression of Atlov1 in Switchgrass Alters Plant Architecture, Lignin Content, and Flowering Time. Plos One, 7(12),en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0047399en
dc.identifier.issn1932-6203en
dc.identifier.issue12en
dc.identifier.urihttp://hdl.handle.net/10919/56679en
dc.identifier.volume7en
dc.language.isoenen
dc.rightsIn Copyrighten
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectArabidopsis-thalianaen
dc.subjectBioenergy productionen
dc.subjectBiofuel productionen
dc.subjectBiomass productionen
dc.subjectFermentable sugar yieldsen
dc.subjectGene encodesen
dc.subjectPanicum-virgatumen
dc.subjectTranscription-factoren
dc.subjectTranslational genomicsen
dc.subjectZinc-finger proteinen
dc.titleOverexpression of AtLOV1 in Switchgrass Alters Plant Architecture, Lignin Content, and Flowering Timeen
dc.title.serialPloS Oneen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
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