IL-12 signaling drives the differentiation and function of a T(H)1-derived T-FH1-like cell population

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dc.contributor.authorPowell, Michael D.en
dc.contributor.authorRead, Kaitlin A.en
dc.contributor.authorSreekumar, Bharath K.en
dc.contributor.authorJones, Devin M.en
dc.contributor.authorOestreich, Kenneth J.en
dc.date.accessioned2019-11-19T14:04:38Zen
dc.date.available2019-11-19T14:04:38Zen
dc.date.issued2019-09-30en
dc.description.abstractCD4(+) T follicular helper (T-FH) cells provide help to B cells and promote antibody-mediated immune responses. Increasing evidence supports the existence of T-FH populations that secrete cytokines typically associated with the effector functions of other CD4(+) T cell subsets. These include T helper 1 (T(H)1)-biased T-FH (T-FH(1)) cells that have recognized roles in both immune responses to pathogens and also the pathogenesis of autoimmune disease. Given their apparent importance to human health, there is interest in understanding the mechanisms that regulate T-FH1 cell formation and function. However, their origin and the molecular requirements for their differentiation are unclear. Here, we describe a population of murineT(H)1-derived,T-FH1-like cells that express the chemokine receptor Cxcr3 and produce both the T(H)1 cytokine interferon-gamma and the T-FH-associated cytokine interleu kin-21 (IL-21). Furthermore, these T-FH1-like cells promote B cell activation and antibody production at levels indistinguishable from conventional IL-6-derived T-FH-like cells. Regarding their regulatory requirements, we find that IL-12 signaling is necessary for the differentiation and function of this T-FH1-like cell population. Specifically, IL-12-dependent activation of STAT4, and unexpectedly STAT3, promotes increased expression of IL-21 and the T-FH lineage-defining transcription factor Bcl-6 in T-FH1-like cells. Taken together, these findings provide insight into the potential origin and differentiation requirements of T-FH1 cells.en
dc.description.notesThis work was supported by grants from The National Institutes of Health grants (AI134972 and AI127800) to K.J.O.en
dc.description.sponsorshipNational Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [AI134972, AI127800]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/s41598-019-50614-1en
dc.identifier.eissn2045-2322en
dc.identifier.other13991en
dc.identifier.pmid31570752en
dc.identifier.urihttp://hdl.handle.net/10919/95804en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIL-12 signaling drives the differentiation and function of a T(H)1-derived T-FH1-like cell populationen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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