Identification and validation of major QTLs associated with low seed coat deficiency of natto soybean seeds (Glycine maxL.)

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dc.contributor.authorZhu, Qianen
dc.contributor.authorEscamilla, Diana M.en
dc.contributor.authorWu, Xingboen
dc.contributor.authorSong, Qijianen
dc.contributor.authorLi, Songen
dc.contributor.authorRosso, M. Lucianaen
dc.contributor.authorLord, Nilankaen
dc.contributor.authorXie, Futien
dc.contributor.authorZhang, Boen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2020-12-15T13:52:08Zen
dc.date.available2020-12-15T13:52:08Zen
dc.date.issued2020-11en
dc.description.abstractKey message Two major QTLs associated with low seed coat deficiency of soybean seeds were identified in two biparental populations, and three SNP markers were validated to assist low-SCD natto soybean breeding selection. Soybean seed coat deficiency (SCD), known as seed coat cracking during soaking in the natto production process, is problematic because split or broken beans clog production lines and increases production costs. Development of natto soybean cultivars with low SCD is crucial to support the growth of the natto industry. Unfortunately, information on the genetic control of SCD in soybean, which is desperately needed to facilitate breeding selection, remains sparse. In this study, two F(2)populations derived from V11-0883 x V12-1626 (Pop 1) and V11-0883 x V12-1885 (Pop 2) were developed and genotyped with BARCSoySNP6K Beadchips and F-2-derived lines were evaluated for SCD in three consecutive years (2016-2018) in order to identify quantitative trait loci (QTLs) associated with low SCD in soybean. A total of 17 QTLs underlying SCD were identified in two populations. Among these, two major and stable QTLs,qSCD15on chromosome 15 andqSCD20on chromosome 20, were detected across multiple years. These QTLs explained up to 30.3% of the phenotypic variation for SCD in Pop 1 and 6.1% in Pop 2 across years. Three SNP markers associated with theqSCD20were validated in additional four biparental populations. The average selection efficiency of low-SCD soybean was 77% based on two tightly linked markers, Gm20_34626867 and Gm20_34942502, and 64% based on the marker Gm20_35625615. The novel and stable QTLs identified in this study will facilitate elucidation of the genetic mechanism controlling SCD in soybean, and the markers will significantly accelerate breeding for low-SCD soybean through marker-assisted selection.en
dc.description.notesThis study was funded by Montague Farms, Inc. (Grant No. 459611) and USDA Hatch Program VA-136296.en
dc.description.sponsorshipMontague Farms, Inc. [459611]; USDA Hatch Program [VA-136296]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s00122-020-03662-5en
dc.identifier.eissn1432-2242en
dc.identifier.issn0040-5752en
dc.identifier.issue11en
dc.identifier.pmid32851437en
dc.identifier.urihttp://hdl.handle.net/10919/101351en
dc.identifier.volume133en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIdentification and validation of major QTLs associated with low seed coat deficiency of natto soybean seeds (Glycine maxL.)en
dc.title.serialTheoretical And Applied Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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