Molecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazil

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dc.contributor.authorFinger, Mariane Angelicaen
dc.contributor.authorBiava, Janaina Socolovskien
dc.contributor.authorDornbusch, Peterson Trichesen
dc.contributor.authorPerotta, Joao Henriqueen
dc.contributor.authorUllmann, Leila Sabrinaen
dc.contributor.authorSerpa, Priscila B. S.en
dc.contributor.authorKmetiuk, Louise Bachen
dc.contributor.authordos Santos, Andrea Piresen
dc.contributor.authorBiondo, Alexander Welkeren
dc.contributor.authorLeutenegger, Christianen
dc.contributor.authorde Barros Filho, Ivan Roqueen
dc.date.accessioned2023-04-25T12:45:33Zen
dc.date.available2023-04-25T12:45:33Zen
dc.date.issued2022-11en
dc.description.abstractBackground and Aim: Molecular approaches to diagnose respiratory viruses have provided an opportunity for early and subclinical pathogen detection, particularly in samples from the upper respiratory tract. This study aimed to investigate the presence of herpesviruses, particularly equid herpesvirus (EHV)-2 and EHV-5, in samples from the lower respiratory tract of healthy racehorses from Southern Brazil. Materials and Methods: Samples from the lower respiratory tract (i.e., bronchoalveolar lavage fluid [BALF]) were assessed by video endoscopy, cytological evaluation of BALF, and tracheal aspirates (TA), along with quantitative polymerase chain reaction (qPCR), to detect equine herpesvirus infection in the lower respiratory tract samples and compare corresponding cytological and endoscopic findings. Results: At least one abnormality per horse during endoscopy examination was observed, including, but not limited to, mucous secretion in the airways and pharyngeal lymphoid hyperplasia. The presence of EHV-2 and/or EHV-5 was detected by qPCR in 3/10 animals. One horse was positive for EHV-2 alone, one for EHV-5 alone, and one for both. Conclusion: To the authors' knowledge, this is the first molecular detection of EHV-2 and EHV-5 in Brazilian thoroughbred horses. These findings may provide new insights into the epidemiology of EHV-2 and EHV-5 in Brazilian horses, evidencing the importance of the molecular investigation, early detection, and prevention of respiratory diseases.en
dc.description.notesThe authors are thankful to State Jockey Club, Paran?, Brazil, or providing the necessary facilities for the study. The authors did not receive any funds for this study.en
dc.description.sponsorshipState Jockey Club, Paranen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.14202/vetworld.2022.2597-2602en
dc.identifier.eissn2231-0916en
dc.identifier.issue11en
dc.identifier.pmid36590117en
dc.identifier.urihttp://hdl.handle.net/10919/114769en
dc.identifier.volume15en
dc.language.isoenen
dc.publisherVeterinary Worlden
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbronchoalveolar lavage fluiden
dc.subjectendoscopyen
dc.subjectequineen
dc.subjectgammaherpesvirusen
dc.subjectpolymerase chain reactionen
dc.subjecttracheal washen
dc.titleMolecular detection of equid herpesvirus in bronchoalveolar lavage fluid from asymptomatic horses in Southern Brazilen
dc.title.serialVeterinary Worlden
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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