Characterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometry

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dc.contributor.authorPierre-Jerome, Edithen
dc.contributor.authorWright, R. Clayen
dc.contributor.authorNemhauser, Jennifer L.en
dc.contributor.departmentBiological Systems Engineeringen
dc.date.accessioned2019-01-21T17:13:22Zen
dc.date.available2019-01-21T17:13:22Zen
dc.date.issued2017-01en
dc.date.updated2019-01-21T17:13:20Zen
dc.description.abstractRecapitulation of the nuclear auxin response pathway in Saccharomyces cerevisiae (yeast) provides a means to functionally assay the contribution of individual signaling components to response dynamics. Here, we describe a time course assay for characterizing auxin response circuits using flow cytometry. This method allows for quantitative measurements of the dynamic response of up to 12 circuits (strains) at once. We also describe a steady-state assay and how to utilize an R package we developed to facilitate data analysis.en
dc.description.versionPublished versionen
dc.format.extentPages 271-281en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/978-1-4939-6469-7_22en
dc.identifier.eissn1940-6029en
dc.identifier.issn1064-3745en
dc.identifier.orcidWright, Robert [0000-0001-7125-3943]en
dc.identifier.otherPMC5269309en
dc.identifier.pmid27864773en
dc.identifier.urihttp://hdl.handle.net/10919/86810en
dc.identifier.volume1497en
dc.language.isoenen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/27864773en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectBudding yeasten
dc.subjectFluorescent reportersen
dc.subjectSignaling dynamicsen
dc.subjectSynthetic biologyen
dc.subject0601 Biochemistry And Cell Biologyen
dc.subjectDevelopmental Biologyen
dc.subject.meshSaccharomyces cerevisiaeen
dc.subject.meshIndoleacetic Acidsen
dc.subject.meshPlant Growth Regulatorsen
dc.subject.meshFlow Cytometryen
dc.subject.meshSignal Transductionen
dc.titleCharacterizing Auxin Response Circuits in Saccharomyces cerevisiae by Flow Cytometryen
dc.title.serialMethods in molecular biology (Clifton, N.J.)en
dc.typeArticleen
dc.type.dcmitypeTexten
dc.type.otherresearch-articleen
dc.type.otherJournal Articleen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biological Systems Engineeringen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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