Computational and Biochemical Analysis of the Xanthomonas Effector AvrBs2 and Its Role in the Modulation of Xanthomonas Type Three Effector Delivery

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dc.contributor.authorZhao, Bingyu Y.en
dc.contributor.authorDahlbeck, Douglasen
dc.contributor.authorKrasileva, Ksenia V.en
dc.contributor.authorFong, Richard W.en
dc.contributor.authorStaskawicz, Brian J.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-12-04T18:42:15Zen
dc.date.available2018-12-04T18:42:15Zen
dc.date.issued2011-12-01en
dc.description.abstractEffectors of the bacterial type III secretion system provide invaluable molecular probes to elucidate the molecular mechanisms of plant immunity and pathogen virulence. In this report, we focus on the AvrBs2 effector protein from the bacterial pathogen Xanthomonas euvesicatoria (Xe), the causal agent of bacterial spot disease of tomato and pepper. Employing homology-based structural analysis, we generate a three-dimensional structural model for the AvrBs2 protein and identify catalytic sites in its putative glycerolphosphodiesterase domain (GDE). We demonstrate that the identified catalytic region of AvrBs2 was able to functionally replace the GDE catalytic site of the bacterial glycerophosphodiesterase BhGlpQ cloned from Borrelia hermsii and is required for AvrBs2 virulence. Mutations in the GDE catalytic domain did not disrupt the recognition of AvrBs2 by the cognate plant resistance gene Bs2. In addition, AvrBs2 activation of Bs2 suppressed subsequent delivery of other Xanthomonas type III effectors into the host plant cells. Investigation of the mechanism underlying this modulation of the type III secretion system may offer new strategies to generate broad-spectrum resistance to bacterial pathogens.en
dc.description.sponsorshipThis work was funded by the Two Blades Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.format.extent13 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1002408en
dc.identifier.eissn1553-7374en
dc.identifier.issn1553-7366en
dc.identifier.issue12en
dc.identifier.othere1002408en
dc.identifier.pmid22144898en
dc.identifier.urihttp://hdl.handle.net/10919/86223en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcampestris pv. vesicatoriaen
dc.subjectnontypable haemophilus-influenzaeen
dc.subjectiii secretion systemsen
dc.subjectbs3 resistance geneen
dc.subjectglycerophosphodiester phosphodiesteraseen
dc.subjectpseudomonas-syringaeen
dc.subjectbacterial spoten
dc.subjectprotein-den
dc.subjectnicotiana-benthamianaen
dc.subjectdisease resistanceen
dc.titleComputational and Biochemical Analysis of the Xanthomonas Effector AvrBs2 and Its Role in the Modulation of Xanthomonas Type Three Effector Deliveryen
dc.title.serialPLOS Pathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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