Pleiotropic Odorant-Binding Proteins Promote Aedes aegypti Reproduction and Flavivirus Transmission

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dc.contributor.authorDong, Shengzhangen
dc.contributor.authorYe, Zien
dc.contributor.authorTikhe, Chinmay Vijayen
dc.contributor.authorTu, Zhijian Jakeen
dc.contributor.authorZwiebel, Laurence J.en
dc.contributor.authorDimopoulos, Georgeen
dc.date.accessioned2022-01-18T02:10:31Zen
dc.date.available2022-01-18T02:10:31Zen
dc.date.issued2021-09-01en
dc.date.updated2022-01-18T02:10:26Zen
dc.description.abstractInsect odorant-binding proteins (OBPs) are small soluble proteins that have been assigned roles in olfaction, but their other potential functions have not been extensively explored. Using CRISPR/Cas9-mediated disruption of Aedes aegypti Obp10 and Obp22, we demonstrate the pleiotropic contribution of these proteins to multiple processes that are essential for vectorial capacity. Mutant mosquitoes have impaired host-seeking and oviposition behavior, reproduction, and arbovirus transmission. Here, we show that Obp22 is linked to the male-determining sex locus (M) on chromosome 1 and is involved in male reproduction, likely by mediating the development of spermatozoa. Although OBP10 and OBP22 are not involved in flavivirus replication, abolition of these proteins significantly reduces transmission of dengue and Zika viruses through a mechanism affecting secretion of viral particles into the saliva. These results extend our current understanding of the role of insect OBPs in insect reproduction and transmission of human pathogens, making them essential determinants of vectorial capacity. IMPORTANCE Aedes aegypti is the major vector for many arthropod-borne viral diseases, such as dengue, Zika, and chikungunya viruses. Previous studies suggested that odorant-binding proteins (OBPs) may have diverse physiological functions beyond the olfactory system in mosquitoes; however, these hypothesized functions have not yet been demonstrated. Here, we have used CRISPR/Cas9-based genome editing to functionally delete (knock out) Obp10 and Obp22 in Aedes aegypti. We showed that disruption of Obp10 or Obp22 significantly impairs female and male reproductive capacity by adversely affecting blood feeding, oviposition, fecundity and fertility, and the development of spermatozoa. We also showed that disruption of Obp10 or Obp22 significantly reduces the transmission of dengue and Zika viruses through a mechanism affecting secretion of viral particles into the saliva. Thus, our study is not only significant in understanding the functions of OBPs in mosquito biology, but also shows that OBPs may represent potent flavivirus transmission-blocking targets. Our study is in this regard particularly timely and important from a translational and public health perspective.en
dc.description.versionPublished versionen
dc.format.extent18 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e02531-21 (Article number)en
dc.identifier.doihttps://doi.org/10.1128/mBio.02531-21en
dc.identifier.eissn2150-7511en
dc.identifier.issn2150-7511en
dc.identifier.issue5en
dc.identifier.orcidTu, Zhijian [0000-0003-4227-3819]en
dc.identifier.pmid34634943en
dc.identifier.urihttp://hdl.handle.net/10919/107712en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000735255900012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectMicrobiologyen
dc.subjectAedes aegyptien
dc.subjectZika virusen
dc.subjectdengue virusen
dc.subjectodorant-binding proteinsen
dc.subjectOBP10en
dc.subjectOBP22en
dc.subjectblood feedingen
dc.subjectfeeding behavioren
dc.subjectReproductionen
dc.subjectvirus transmissionen
dc.subjectAnopheles-Gambiaeen
dc.subjectCrystal-Structureen
dc.subjectMosquitoen
dc.subjectIdentificationen
dc.subjectReceptorsen
dc.subjectGlanden
dc.subject0605 Microbiologyen
dc.titlePleiotropic Odorant-Binding Proteins Promote Aedes aegypti Reproduction and Flavivirus Transmissionen
dc.title.serialmBioen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten

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