Metagenomic profiling of historic Colorado Front Range flood impact on distribution of riverine antibiotic resistance genes

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dc.contributor.authorGarner, Emilyen
dc.contributor.authorWallace, Joshua S.en
dc.contributor.authorArango-Argoty, Gustavoen
dc.contributor.authorWilkinson, Caitlinen
dc.contributor.authorFahrenfeld, Nicoleen
dc.contributor.authorHeath, Lenwood S.en
dc.contributor.authorZhang, Liqingen
dc.contributor.authorArabi, Mazdaken
dc.contributor.authorAga, Diana S.en
dc.contributor.authorPruden, Amyen
dc.contributor.departmentCivil and Environmental Engineeringen
dc.contributor.departmentComputer Scienceen
dc.coverage.stateColoradoen
dc.date.accessioned2017-03-08T19:40:30Zen
dc.date.available2017-03-08T19:40:30Zen
dc.date.issued2016-12-05en
dc.description.abstractRecord-breaking floods in September 2013 caused massive damage to homes and infrastructure across the Colorado Front Range and heavily impacted the Cache La Poudre River watershed. Given the unique nature of this watershed as a test-bed for tracking environmental pathways of antibiotic resistance gene (ARG) dissemination, we sought to determine the impact of extreme flooding on ARG reservoirs in river water and sediment. We utilized high-throughput DNA sequencing to obtain metagenomic profiles of ARGs before and after flooding, and investigated 23 antibiotics and 14 metals as putative selective agents during post-flood recovery. With 277 ARG subtypes identified across samples, total bulk water ARGs decreased following the flood but recovered to near pre-flood abundances by ten months post-flood at both a pristine site and at a site historically heavily influenced by wastewater treatment plants and animal feeding operations. Network analysis of de novo assembled sequencing reads into 52,556 scaffolds identified ARGs likely located on mobile genetic elements, with up to 11 ARGs per plasmid-associated scaffold. Bulk water bacterial phylogeny correlated with ARG profiles while sediment phylogeny varied along the river’s anthropogenic gradient. This rare flood afforded the opportunity to gain deeper insight into factors influencing the spread of ARGs in watersheds.en
dc.description.versionPublished versionen
dc.format.extent? - ? (10) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1038/srep38432en
dc.identifier.issn2045-2322en
dc.identifier.orcidHeath, LS [0000-0003-1608-431X]en
dc.identifier.urihttp://hdl.handle.net/10919/75499en
dc.identifier.volume6en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000389190100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectmetal resistanceen
dc.subjectsequencing dataen
dc.subjectwaste-wateren
dc.subjectdatabaseen
dc.subjectsoilen
dc.subjecttetracyclineen
dc.subjectenvironmenten
dc.subjectcoselectionen
dc.subjectbacteriaen
dc.subjectmanureen
dc.titleMetagenomic profiling of historic Colorado Front Range flood impact on distribution of riverine antibiotic resistance genesen
dc.title.serialScientific Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Civil & Environmental Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/Computer Scienceen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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