Identification of the Target for a Transition Metal-alpha-Amino Acid Complex Antibiotic Against Mycobacterium smegmatis

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dc.contributor.authorKarpin, George W.en
dc.contributor.authorMerola, Joseph S.en
dc.contributor.authorFalkinham, Joseph O. IIIen
dc.date.accessioned2022-04-19T14:42:14Zen
dc.date.available2022-04-19T14:42:14Zen
dc.date.issued2021-06-25en
dc.description.abstractSpontaneous mutants of Mycobacterium smegmatis strain mc(2)155 resistant to 1-PG (iridium-L-phenylglycine complex), an antimycobacterial antibiotic, were isolated. Based on the discovery that some 1-PG-resistant mutants (1-PG(R)) were also resistant to high concentrations of clarithromycin (>= 250 mu g/ml), but no other anti-mycobacterial antibiotics, the 23S rRNA region spanning the peptidyl transferase domain was sequenced and mutations shown to be localized in the peptidyl transferase domain of the 23S rRNA gene. Measurements showed that 1-PG bound to ribosomes isolated from the 1-PG-sensitive parental strain, but the ribosome binding values for the 1-PG(R) mutant reduced.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphar.2021.686358en
dc.identifier.issn1663-9812en
dc.identifier.other686358en
dc.identifier.pmid34248636en
dc.identifier.urihttp://hdl.handle.net/10919/109695en
dc.identifier.volume12en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecttransition metal-alpha-amino acid complexesen
dc.subjectmycobacteriaen
dc.subjectclarithromycinen
dc.subject23S rRNAen
dc.subjectpeptidyl transferaseen
dc.titleIdentification of the Target for a Transition Metal-alpha-Amino Acid Complex Antibiotic Against Mycobacterium smegmatisen
dc.title.serialFrontiers in Pharmacologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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