Profiling renal dysfunction using Raman chemometric urinalysis, with special reference to COVID19, lupus nephritis, and diabetic nephropathy

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dc.contributor.authorRobertson, John L.en
dc.contributor.authorIssa, Amr Sayeden
dc.contributor.authorGomez, Marianaen
dc.contributor.authorSullivan, Kathleenen
dc.contributor.authorSenger, Ryan S.en
dc.date.accessioned2023-10-26T17:19:45Zen
dc.date.available2023-10-26T17:19:45Zen
dc.date.issued2023-09-30en
dc.date.updated2023-10-26T17:14:58Zen
dc.description.abstractBackground: Many systemic and urinary tract diseases alter renal structure and function, including changing the composition of urine. While routine urinalysis (physical properties, sediment evaluation, urine chemistry analytes) is useful in screening, it has limitations on separating disease processes, structural changes, and functional abnormalities. Likewise, while many individual ‘biomarkers’ have been used to screen for disease, they have not met with widespread clinical adoption. The recent COVID19 Pandemic and the recognition of post-acute sequelae SARS-CoV-2 infection (PASC) have highlighted the need for rapid, scalable, economical, and accurate screening tools for managing disease. Aims: Validate a Raman spectroscopy-based screening technology for urine analysis that could be used for recognition and quantification of systemic and renal effects of acute and PASC COVID19 disease. Methods: One hundred ten (110) urine specimens were obtained from consented adults diagnosed with COVID19 disease by RT-PCR and/or proximate (household) contact With RT-PCR-confirmed COVID19 disease. Samples were analyzed using Raman chemometric urinalysis, a technology that detects hundreds of discrete chemicals in urine and applies computational comparison-machine learning to detect COVID19-associated molecular patterns (‘fingerprints’). Results: When compared with the urine multimolecular ‘fingerprints’ of healthy individuals and patients with known systemic diseases (diabetes mellitus, lupus) that alter renal structure and function, patients with acute and PASC COVID19 had unique ‘fingerprints’ indicative of alterations in renal function (i.e. – infection altered urine composition). Differences in disease severity (mild to severe) were reflected by different ‘fingerprints’ in urine. Roughly 20% of hospitalized patients developed a degree of renal dysfunction (decrements in eGFR) that were correlated with distinct changes in urine fingerprints. Conclusion: Raman chemometric urinalysis may be a useful tool in management of patients with COVID19 disease, particularly in detecting patients with evolving renal dysfunction for whom there should be attention to medication use and renal health restoration/preservation.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.18103/mra.v11i9.4384en
dc.identifier.eissn2375-1924en
dc.identifier.issn2375-1916en
dc.identifier.issue9en
dc.identifier.orcidGomez, Mariana [0000-0001-8963-9959]en
dc.identifier.urihttp://hdl.handle.net/10919/116550en
dc.identifier.volume11en
dc.language.isoenen
dc.publisherKnowledge Enterprise Journalsen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject32 Biomedical and Clinical Sciencesen
dc.subject3202 Clinical Sciencesen
dc.subject34 Chemical Sciencesen
dc.subjectLupusen
dc.subjectPreventionen
dc.subjectClinical Researchen
dc.subjectAutoimmune Diseaseen
dc.subjectKidney Diseaseen
dc.subject4.1 Discovery and preclinical testing of markers and technologiesen
dc.subject4 Detection, screening and diagnosisen
dc.subject4.2 Evaluation of markers and technologiesen
dc.titleProfiling renal dysfunction using Raman chemometric urinalysis, with special reference to COVID19, lupus nephritis, and diabetic nephropathyen
dc.title.serialMedical Research Archivesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicine/Infectious Diseaseen

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