Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding
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TR Number
Date
2014-08-08
Journal Title
Journal ISSN
Volume Title
Publisher
The Royal Society of Chemistry
Abstract
Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.
Description
Keywords
Fluorescence resonance energy transfer, Biosensors, Genetic encoding
Citation
Sun, C., Ouyang, M., Cao, Z., Ma, S., Alqublan, H., Sriranganathan, N., Wang, Y., Lu, C. (2014). Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chemical Communications, 50(78), 11536-11539. doi: 10.1039/C4CC04730C