Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

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dc.contributorVirginia Tech. School of Biomedical Engineering and Sciencesen
dc.contributorUniversity of California, San Diego. Department of Bioengineering and Institute of Engineering in Medicineen
dc.contributorVirginia Tech. Department of Biomedical Sciences & Pathobiologyen
dc.contributorVirginia Tech. Department of Chemical Engineeringen
dc.contributor.authorSun, Chenen
dc.contributor.authorOuyang, Mingxingen
dc.contributor.authorCao, Zhenningen
dc.contributor.authorMa, Saien
dc.contributor.authorAlqublan, Hamzehen
dc.contributor.authorSriranganathan, Nammalwaren
dc.contributor.authorWang, Yingxiaoen
dc.contributor.authorLu, Changen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessed2015-04-16en
dc.date.accessioned2015-04-20T22:22:11Zen
dc.date.available2015-04-20T22:22:11Zen
dc.date.issued2014-08-08en
dc.description.abstractFluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ~100 cells with high spatiotemporal resolution.en
dc.description.notesSupplementary information is included in a separate fileen
dc.description.sponsorshipVirginia Tech. Institute for Critical Technology and Applied Scienceen
dc.description.sponsorshipNational Science Foundation (U.S.) - Grant 1016547en
dc.description.sponsorshipNational Science Foundation (U.S.) - Grant 0967069en
dc.description.sponsorshipNational Science Foundation (U.S.) - Grant 1344298en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationSun, C., Ouyang, M., Cao, Z., Ma, S., Alqublan, H., Sriranganathan, N., Wang, Y., Lu, C. (2014). Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding. Chemical Communications, 50(78), 11536-11539. doi: 10.1039/C4CC04730Cen
dc.identifier.doihttps://doi.org/10.1039/C4CC04730Cen
dc.identifier.issn1359-7345en
dc.identifier.urihttp://hdl.handle.net/10919/51723en
dc.identifier.urlhttp://pubs.rsc.org/en/content/articlelanding/2014/cc/c4cc04730cen
dc.language.isoenen
dc.publisherThe Royal Society of Chemistryen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectFluorescence resonance energy transferen
dc.subjectBiosensorsen
dc.subjectGenetic encodingen
dc.titleElectroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encodingen
dc.title.serialChemical Communicationsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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