A chromosome 16 deletion conferring a high sucrose phenotype in soybean

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dc.contributor.authorOstezan, Alexandraen
dc.contributor.authorPrenger, Elizabeth M.en
dc.contributor.authorRosso, Lucianaen
dc.contributor.authorZhang, Boen
dc.contributor.authorStupar, Robert M.en
dc.contributor.authorGlenn, Travisen
dc.contributor.authorMian, M. A. Roufen
dc.contributor.authorLi, Zengluen
dc.date.accessioned2023-05-22T17:46:35Zen
dc.date.available2023-05-22T17:46:35Zen
dc.date.issued2023-05en
dc.description.abstractKey messageSucrose in soybean seeds is desirable for many end-uses. Increased sucrose contents were discovered to associate with a chromosome 16 deletion resulting from fast neutron irradiation.Soybean is one of the most economically important crops in the United States. A primary end-use of soybean is for livestock feed. Therefore, genetic improvement of seed composition is one of the most important goals in soybean breeding programs. Sucrose is desired in animal feed due to its role as an easily digestible energy source. An elite soybean line was irradiated with fast neutrons and the seed from plants were screened for altered seed composition with near-infrared spectroscopy (NIR). One mutant line, G15FN-54, was found to have higher sucrose content (8-9%) than the parental line (5-6%). Comparative genomic hybridization (CGH) revealed three large deletions on chromosomes (Chrs) 10, 13, and 16 in the mutant, which were confirmed through whole genome sequencing (WGS). A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype. The mapping result using the bi-parental population confirmed that the deletion on Chr 16 conferred elevated sucrose content and a total of 21 genes are located within this Chr 16 deletion. NIR and high-pressure liquid chromatography (HPLC) were used to confirm the stability of the phenotype across generations in the bi-parental population. The mutation will be useful to understand the genetic control of soybean seed sucrose content.en
dc.description.adminPublic domain – authored by a U.S. government employeeen
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s00122-023-04354-6en
dc.identifier.eissn1432-2242en
dc.identifier.issn0040-5752en
dc.identifier.issue5en
dc.identifier.pmid37039870en
dc.identifier.urihttp://hdl.handle.net/10919/115137en
dc.identifier.volume136en
dc.language.isoenen
dc.publisherSpringeren
dc.rightsPublic Domain (U.S.)en
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subjectFast-neutron irradiationen
dc.subjectseed proteinen
dc.subjectcarbohydrate profilesen
dc.subjectoil contenten
dc.subjectarabidopsisen
dc.subjectraffinoseen
dc.subjectmutanten
dc.subjectoligosaccharideen
dc.subjectidentificationen
dc.subjectstarchen
dc.titleA chromosome 16 deletion conferring a high sucrose phenotype in soybeanen
dc.title.serialTheoretical and Applied Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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