Cancer Cells Sense Fibers by Coiling on them in a Curvature-Dependent Manner

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dc.contributor.authorMukherjee, Apratimen
dc.contributor.authorBehkam, Baharehen
dc.contributor.authorNain, Amrinder S.en
dc.contributor.departmentMechanical Engineeringen
dc.date.accessioned2020-02-18T18:49:51Zen
dc.date.available2020-02-18T18:49:51Zen
dc.date.issued2019-09-27en
dc.description.abstractMetastatic cancer cells sense the complex and heterogeneous fibrous extracellular matrix (ECM) by formation of protrusions, and our knowledge of how cells physically recognize these fibers remains in its infancy. Here, using suspended ECM-mimicking isodiameter fibers ranging from 135 to 1,000 nm, we show that metastatic breast cancer cells sense fiber diameters differentially by coiling (wrapping-around) on them in a curvature-dependent manner, whereas non-tumorigenic cells exhibit diminished coiling. We report that coiling occurs at the tip of growing protrusions and the coil width and coiling rate increase in a curvature-dependent manner, but time to maximum coil width occurs biphasically. Interestingly, bundles of 135-nm diameter fibers recover coiling width and rate on 1,000-nm-diameter fibers. Coiling also coincides with curvature-dependent persistent and ballistic transport of endogenous granules inside the protrusions. Altogether, our results lay the groundwork to link biophysical sensing with biological signaling to quantitate pro- and anti-invasive fibrous environments.en
dc.description.notesThis work is supported by NSF (1437101 and 1462916) awarded to A.S.N. and NSF CAREER (1454226) to B.B. Authors acknowledge the support from Institute for Critical Technology and Applied Science (ICTAS) and Macromolecules Innovative Institute, Virginia Tech. A.M. and A.S.N. would like to thank members of the STEP Lab, Virginia Tech, for their helpful suggestions and discussions. The authors thank Professor Aime Franco (University of Arkansas) for providing thyroid cancer cells.en
dc.description.sponsorshipNSFNational Science Foundation (NSF) [1437101, 1462916]; NSF CAREERNational Science Foundation (NSF)NSF - Office of the Director (OD) [1454226]; Institute for Critical Technology and Applied Science (ICTAS); Macromolecules Innovative Institute, Virginia Techen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.isci.2019.08.023en
dc.identifier.issn2589-0042en
dc.identifier.pmid31513975en
dc.identifier.urihttp://hdl.handle.net/10919/96924en
dc.identifier.volume19en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.titleCancer Cells Sense Fibers by Coiling on them in a Curvature-Dependent Manneren
dc.title.serialIscienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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