Probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR gamma to suppress colitis

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dc.contributor.authorBassaganya-Riera, Josepen
dc.contributor.authorViladomiu, Monicaen
dc.contributor.authorPedragosa, Mireiaen
dc.contributor.authorDe Simone, Claudioen
dc.contributor.authorCarbo, Adriaen
dc.contributor.authorShaykhutdinov, Rustemen
dc.contributor.authorJobin, Christianen
dc.contributor.authorArthur, Janelle C.en
dc.contributor.authorCorl, Benjamin A.en
dc.contributor.authorVogel, Hansen
dc.contributor.authorStorr, Martinen
dc.contributor.authorHontecillas, Raquelen
dc.contributor.departmentDairy Scienceen
dc.contributor.departmentFralin Life Sciences Instituteen
dc.date.accessed2014-04-30en
dc.date.accessioned2014-06-17T20:12:07Zen
dc.date.available2014-06-17T20:12:07Zen
dc.date.issued2012-02-21en
dc.description.abstractBackground: Inflammatory bowel disease (IBD) therapies are modestly successful and associated with significant side effects. Thus, the investigation of novel approaches to prevent colitis is important. Probiotic bacteria can produce immunoregulatory metabolites in vitro such as conjugated linoleic acid (CLA), a polyunsaturated fatty acid with potent anti-inflammatory effects. This study aimed to investigate the cellular and molecular mechanisms underlying the anti-inflammatory efficacy of probiotic bacteria using a mouse model of colitis. Methodology/Principal Findings: The immune modulatory mechanisms of VSL#3 probiotic bacteria and CLA were investigated in a mouse model of DSS colitis. Colonic specimens were collected for histopathology, gene expression and flow cytometry analyses. Immune cell subsets in the mesenteric lymph nodes (MLN), spleen, blood and colonic lamina propria cells were phenotypically and functionally characterized. Fecal samples and colonic contents were collected to determine the effect of VSL#3 and CLA on gut microbial diversity and CLA production. CLA and VSL#3 treatment ameliorated colitis and decreased colonic bacterial diversity, a finding that correlated with decreased gut pathology. Colonic CLA concentrations were increased in response to probiotic bacterial treatment, but without systemic distribution in blood. VSL#3 and CLA decreased macrophage accumulation in the MLN of mice with DSS colitis. The loss of PPAR γ in myeloid cells abrogated the protective effect of probiotic bacteria and CLA in mice with DSS colitis. Conclusions/Significance: Probiotic bacteria modulate gut microbial diversity and favor local production of CLA in the colon that targets myeloid cell PPAR γ to suppress colitis.en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBassaganya-Riera J, Viladomiu M, Pedragosa M, De Simone C, Carbo A, et al. (2012) Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis. PLoS ONE 7(2): e31238. doi:10.1371/journal.pone.0031238en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0031238en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/10919/48990en
dc.identifier.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031238en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectBacterial pathologyen
dc.subjectColonen
dc.subjectGut Bacteriaen
dc.subjectInflamationen
dc.subjectLinoleic aciden
dc.subjectMicrophagesen
dc.subjectProbioticsen
dc.subjectColitisen
dc.titleProbiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR gamma to suppress colitisen
dc.title.serialPLoS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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