Heat Shock Proteins in Relation to Heat Stress Tolerance of Creeping Bentgrass at Different N Levels

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dc.contributor.authorWang, Kehuaen
dc.contributor.authorZhang, Xunzhongen
dc.contributor.authorGoatley, Mikeen
dc.contributor.authorErvin, Erik H.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-10-04T14:11:22Zen
dc.date.available2018-10-04T14:11:22Zen
dc.date.issued2014-07-22en
dc.description.abstractHeat stress is a primary factor causing summer bentgrass decline. Changes in gene expression at the transcriptional and/or translational level are thought to be a fundamental mechanism in plant response to environmental stresses. Heat stress redirects protein synthesis in higher plants and results in stress protein synthesis, particularly heat shock proteins (HSPs). The goal of this work was to analyze the expression pattern of major HSPs in creeping bentgrass (Agrostis stolonifera L.) during different heat stress periods and to study the influence of nitrogen (N) on the HSP expression patterns. A growth chamber study on ‘Penn-A4’ creeping bentgrass subjected to 38/28°C day/night for 50 days, was conducted with four nitrate rates (no N-0, low N-2.5, medium N-7.5, and high N-12.5 kg N ha−1) applied biweekly. Visual turfgrass quality (TQ), normalized difference vegetation index (NDVI), photochemical efficiency of photosystem II (Fv/Fm), shoot electrolyte leakage (ShEL), and root viability (RV) were monitored, along with the expression pattern of HSPs. There was no difference in measured parameters between treatments until week seven, except TQ at week five. At week seven, grass at medium N had better TQ, NDVI, and Fv/Fm accompanied by lower ShEL and higher RV, suggesting a major role in improved heat tolerance. All the investigated HSPs (HSP101, HSP90, HSP70, and sHSPs) were up-regulated by heat stress. Their expression patterns indicated cooperation between different HSPs and their roles in bentgrass thermotolerance. In addition, their production seems to be resource dependent. This study could further improve our understanding about how different N levels affect bentgrass thermotolerance.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0102914en
dc.identifier.eissn1932-6203en
dc.identifier.issue7en
dc.identifier.othere102914en
dc.identifier.pmid25050702en
dc.identifier.urihttp://hdl.handle.net/10919/85225en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherPLOSen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleHeat Shock Proteins in Relation to Heat Stress Tolerance of Creeping Bentgrass at Different N Levelsen
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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