Elucidation of an oral drug delivery mechanism to the mammalian gastrointestinal tract via an engineered T4 phage
dc.contributor.author | Baker, Zachary Robert | en |
dc.contributor.committeechair | Hsu, Bryan | en |
dc.contributor.committeemember | Stevens, Ann M. | en |
dc.contributor.committeemember | Caswell, Clayton Christopher | en |
dc.contributor.committeemember | Li, Liwu | en |
dc.contributor.department | Biological Sciences | en |
dc.date.accessioned | 2025-05-14T08:01:07Z | en |
dc.date.available | 2025-05-14T08:01:07Z | en |
dc.date.issued | 2025-05-13 | en |
dc.description.abstractgeneral | Current treatments of gastrointestinal (gut) disease are limited by high dosing frequency, patient noncompliance, and complications circumventing the harsh conditions of the upper gastrointestinal tract (e.g. stomach), leading to therapeutic degradation. Bacterial cells were previously explored as vectors for biologic delivery, yet cellular engineering approaches have traditionally lowered fitness and colonization potential of these microbial factories. As a result, recombinant bacteria are often outcompeted by the native gut flora, requiring high concentrations to alleviate disease pathology. These limitations support the need for novel mechanisms of treatment that improve oral delivery compliance and ease of use. Through the research described in this dissertation, we outline the potential to use bacteriophage as an alternative chassis for biologic production and delivery to the gastrointestinal tract, targeting and reducing symptoms of obesity and ulcerative colitis. Elucidation of this system presents a universal platform for the long-term amelioration of gastrointestinal disease through the use of lytic phage particles. | en |
dc.description.degree | Doctor of Philosophy | en |
dc.format.medium | ETD | en |
dc.identifier.other | vt_gsexam:43391 | en |
dc.identifier.uri | https://hdl.handle.net/10919/132452 | en |
dc.language.iso | en | en |
dc.publisher | Virginia Tech | en |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.subject | Bacteriophage | en |
dc.subject | Intestinal microbiome | en |
dc.subject | Drug delivery | en |
dc.subject | Phage engineering | en |
dc.subject | Obesity | en |
dc.subject | Inflammatory Bowel Disease | en |
dc.title | Elucidation of an oral drug delivery mechanism to the mammalian gastrointestinal tract via an engineered T4 phage | en |
dc.type | Dissertation | en |
thesis.degree.discipline | Biological Sciences | en |
thesis.degree.grantor | Virginia Polytechnic Institute and State University | en |
thesis.degree.level | doctoral | en |
thesis.degree.name | Doctor of Philosophy | en |
Files
Original bundle
1 - 5 of 9
- Name:
- Baker_ZR_D_2025_support_6.pdf
- Size:
- 140.34 KB
- Format:
- Adobe Portable Document Format
- Description:
- Supporting documents
- Name:
- Baker_ZR_D_2025_support_4.pdf
- Size:
- 81.31 KB
- Format:
- Adobe Portable Document Format
- Description:
- Supporting documents
- Name:
- Baker_ZR_D_2025_support_1.pdf
- Size:
- 142.57 KB
- Format:
- Adobe Portable Document Format
- Description:
- Supporting documents
- Name:
- Baker_ZR_D_2025_support_3.pdf
- Size:
- 99.44 KB
- Format:
- Adobe Portable Document Format
- Description:
- Supporting documents