Nitrite reductase activity in F420-dependent sulphite reductase (Fsr) from Methanocaldococcus jannaschii
| dc.contributor.author | Heryakusuma, Christian | en |
| dc.contributor.author | Johnson, Eric F. | en |
| dc.contributor.author | Purwantini, Endang | en |
| dc.contributor.author | Mukhopadhyay, Biswarup | en |
| dc.date.accessioned | 2024-01-17T20:26:30Z | en |
| dc.date.available | 2024-01-17T20:26:30Z | en |
| dc.date.issued | 2023-04-20 | en |
| dc.description.abstract | <i>Methanocaldococcus jannaschii</i> (<i>Mj</i>), a hyperthermophilic and evolutionarily deeply rooted methanogenic archaeon from a deep-sea hydrothermal vent, produces F<sub>420</sub>-dependent sulphite reductase (Fsr) in response to exposure to sulphite. This enzyme allows <i>Mj</i> to detoxify sulphite, a potent inhibitor of methyl coenzyme-M reductase (Mcr), by reducing it to sulphide with reduced coenzyme F<sub>420</sub> (F<sub>420</sub>H<sub>2</sub>) as an electron donor; Mcr is essential for energy production for a methanogen. Fsr allows <i>Mj</i> to utilize sulphite as a sulphur source. Nitrite is another potent inhibitor of Mcr and is toxic to methanogens. It is reduced by most sulphite reductases. In this study, we report that <i>Mj</i>Fsr reduced nitrite to ammonia with F<sub>420</sub>H<sub>2</sub> with physiologically relevant <i>K</i> <sub>m</sub> values (nitrite, 8.9 µM; F<sub>420</sub>H<sub>2</sub>, 9.7 µM). The enzyme also reduced hydroxylamine with a <i>K</i> <sub>m</sub> value of 112.4 µM, indicating that it was an intermediate in the reduction of nitrite to ammonia. These results open the possibility that <i>Mj</i> could use nitrite as a nitrogen source if it is provided at a low concentration of the type that occurs in its habitat. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.doi | https://doi.org/10.1099/acmi.0.000482.v3 | en |
| dc.identifier.eissn | 2516-8290 | en |
| dc.identifier.issn | 2516-8290 | en |
| dc.identifier.issue | 4 | en |
| dc.identifier.orcid | Purwantini, Endang [0000-0002-4113-9995] | en |
| dc.identifier.orcid | Mukhopadhyay, Biswarup [0000-0003-0736-0298] | en |
| dc.identifier.other | PMC10202398 | en |
| dc.identifier.other | 000482.v3 (PII) | en |
| dc.identifier.pmid | 37223055 | en |
| dc.identifier.uri | https://hdl.handle.net/10919/117388 | en |
| dc.identifier.volume | 5 | en |
| dc.language.iso | en | en |
| dc.publisher | Microbiology Society | en |
| dc.relation.uri | https://www.ncbi.nlm.nih.gov/pubmed/37223055 | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | F420-dependent nitrite reductase (FNiR) | en |
| dc.subject | F420-dependent sulphite reductase (Fsr) | en |
| dc.subject | F420H2 | en |
| dc.subject | coenzyme F420 | en |
| dc.subject | deazaflavin | en |
| dc.subject | methanogen | en |
| dc.title | Nitrite reductase activity in F<sub>420</sub>-dependent sulphite reductase (Fsr) from <i>Methanocaldococcus jannaschii</i> | en |
| dc.title.serial | Access Microbiology | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |
| dc.type.other | research-article | en |
| dc.type.other | Journal Article | en |
| dcterms.dateAccepted | 2023-01-25 | en |
| pubs.organisational-group | /Virginia Tech | en |
| pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences | en |
| pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Biochemistry | en |
| pubs.organisational-group | /Virginia Tech/Faculty of Health Sciences | en |
| pubs.organisational-group | /Virginia Tech/All T&R Faculty | en |
| pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty | en |
| pubs.organisational-group | /Virginia Tech/VT Carilion School of Medicine | en |
| pubs.organisational-group | /Virginia Tech/VT Carilion School of Medicine/Internal Medicine | en |
| pubs.organisational-group | /Virginia Tech/VT Carilion School of Medicine/Internal Medicine/Secondary Appointment- Internal Medicine | en |
| pubs.organisational-group | /Virginia Tech/VT Carilion School of Medicine/Internal Medicine/Internal Med-Subgroup | en |