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High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials

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dc.contributor.authorSolares, Maria J.en
dc.contributor.authorJonaid, G. M.en
dc.contributor.authorLuqiu, William Y.en
dc.contributor.authorBerry, Samanthaen
dc.contributor.authorKhadela, Jankien
dc.contributor.authorLiang, Yanpingen
dc.contributor.authorEvans, Madison C.en
dc.contributor.authorPridham, Kevin J.en
dc.contributor.authorDearnaley, William J.en
dc.contributor.authorSheng, Zhien
dc.contributor.authorKelly, Deborah F.en
dc.date.accessioned2024-08-30T19:22:39Zen
dc.date.available2024-08-30T19:22:39Zen
dc.date.issued2022-07-14en
dc.description.abstractMutations in tumor suppressor genes, such as Tumor Protein 53 (TP53), are heavily implicated in aggressive cancers giving rise to gain- and loss-of-function phenotypes. While individual domains of the p53 protein have been studied extensively, structural information for full-length p53 remains incomplete. Functionalized microprocessor chips (microchips) with properties amenable to electron microscopy permitted us to visualize complete p53 assemblies for the first time. The new structures revealed p53 in an inactive dimeric state independent of DNA binding. Residues located at the protein-protein interface corresponded with modification sites in cancer-related hot spots. Changes in these regions may amplify the toxic effects of clinical mutations. Taken together, these results contribute advances in technology and imaging approaches to decode native protein models in different states of activation.en
dc.description.versionAccepted versionen
dc.format.extent10 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e202200310 (Article number)en
dc.identifier.doihttps://doi.org/10.1002/cbic.202200310en
dc.identifier.eissn1439-7633en
dc.identifier.issn1439-4227en
dc.identifier.issue17en
dc.identifier.orcidSheng, Zhi [0000-0002-0029-8666]en
dc.identifier.pmid35789183en
dc.identifier.urihttps://hdl.handle.net/10919/121042en
dc.identifier.volume23en
dc.language.isoenen
dc.publisherWiley-V C H en
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/35789183en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectcanceren
dc.subjectelectron microscopyen
dc.subjectmicrochipsen
dc.subjectp53en
dc.subjectmolecular modelingen
dc.subject.meshHumansen
dc.subject.meshNeoplasmsen
dc.subject.meshMutationen
dc.subject.meshMicrocomputersen
dc.subject.meshTumor Suppressor Protein p53en
dc.titleHigh-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materialsen
dc.title.serialChemBioChemen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Faculty of Health Sciencesen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicine/Secondary Appointment- Internal Medicineen
pubs.organisational-groupVirginia Tech/VT Carilion School of Medicine/Internal Medicine/Internal Med-Subgroupen
pubs.organisational-groupVirginia Tech/University Research Institutesen
pubs.organisational-groupVirginia Tech/University Research Institutes/Fralin Biomedical Research Institute at VTCen

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