Cytokine mRNA Expression in the Small Intestine of Weanling Pigs Fed Diets Supplemented with Specialized Protein or Peptide Sources

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dc.contributor.authorZhao, J.en
dc.contributor.authorHarper, Allen F.en
dc.contributor.authorWebb, Kenneth E. Jr.en
dc.contributor.authorKuehn, Larry Alexanderen
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.authorXiao, X.en
dc.contributor.authorWong, Eric A.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2020-01-02T19:30:32Zen
dc.date.available2020-01-02T19:30:32Zen
dc.date.issued2008-12en
dc.description.abstractCytokines play a central role in the mucosal immune response and are involved in regulation of nutrient absorption, metabolism and animal growth This study investigated the effect of diet manipulation with specialized protein or peptide sources on expression of cytokine (IL-1, IL-6, IL-10, and TNF-alpha) mRNA abundance in different intestinal regions and at different ages post-weaning in piglets. A total of 48 (17 days of age, 6.16 +/- 0.34 kg BW) weanling pigs were fed either a corn-soy/whey protein basal diet, the basal diet supplemented with spray-dried plasma protein (SDPP), or the basal diet supplemented with Peptiva (R), a hydrolyzed marine plant protein. A fourth treatment group was fed the SDPP diet, but the feed intake level was limited (SDPP-LF). Pigs were killed at 3 and 10 d, and intestinal cytokine mRNA was measured by real-time PCR using the relative quantification method. The SDPP-LF group exhibited an increased TNF-alpha mRNA abundance compared with the ad libitum SDPP group (p<0.05). The TNF-alpha and IL-10 mRNA abundance increased from the proximal to distal part of the intestine, and the mRNA abundance was greater (p<0.01) in the distal intestine as compared with the proximal and middle intestine. The cytokines IL-1-beta, IL-10 and TNF-alpha mRNA abundance also increased from d3 to d10 postweaning (p<0.01). In summary, restricted feeding increased the TNF-alpha mRNA abundance in the small intestine, however neither SDPP nor peptide supplementation affected cytokine mRNA expression. Abundance of mRNA for most cytokines examined in this study increased with age post-weaning, suggesting that during 10 d after weaning the mucosal immune system is still under development.en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.5713/ajas.2008.80134en
dc.identifier.eissn1976-5517en
dc.identifier.issn1011-2367en
dc.identifier.issue12en
dc.identifier.urihttp://hdl.handle.net/10919/96255en
dc.identifier.volume21en
dc.language.isoenen
dc.rightsCreative Commons Attribution-NonCommercial 3.0 Unporteden
dc.rights.urihttp://creativecommons.org/licenses/by-nc/3.0/en
dc.subjectCytokineen
dc.subjectPeptideen
dc.subjectPigen
dc.subjectReal-time PCRen
dc.subjectSpray-dried Plasma Proteinen
dc.titleCytokine mRNA Expression in the Small Intestine of Weanling Pigs Fed Diets Supplemented with Specialized Protein or Peptide Sourcesen
dc.title.serialAsian-Australasian Journal of Animal Sciencesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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