Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space

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dc.contributor.authorMassa, Silviaen
dc.contributor.authorPagliarello, Riccardoen
dc.contributor.authorCemmi, Alessiaen
dc.contributor.authorDi Sarcina, Ilariaen
dc.contributor.authorBombarely, Aurelianoen
dc.contributor.authorDemurtas, Olivia Costantinaen
dc.contributor.authorDiretto, Gianfrancoen
dc.contributor.authorPaolini, Francescaen
dc.contributor.authorPetzold, H. Earlen
dc.contributor.authorBliek, Mattijsen
dc.contributor.authorBennici, Elisabettaen
dc.contributor.authorDel Fiore, Antonellaen
dc.contributor.authorDe Rossi, Patriziaen
dc.contributor.authorSpelt, Cornelisen
dc.contributor.authorKoes, Ronalden
dc.contributor.authorQuattrocchio, Francescaen
dc.contributor.authorBenvenuto, Eugenioen
dc.date.accessioned2022-07-22T16:58:41Zen
dc.date.available2022-07-22T16:58:41Zen
dc.date.issued2022-02-24en
dc.description.abstractGene expression manipulation of specific metabolic pathways can be used to obtain bioaccumulation of valuable molecules and desired quality traits in plants. A single-gene approach to impact different traits would be greatly desirable in agrospace applications, where several aspects of plant physiology can be affected, influencing growth. In this work, MicroTom hairy root cultures expressing a MYB-like transcription factor that regulates the biosynthesis of anthocyanins in Petunia hybrida (PhAN4), were considered as a testbed for bio-fortified tomato whole plants aimed at agrospace applications. Ectopic expression of PhAN4 promoted biosynthesis of anthocyanins, allowing to profile 5 major derivatives of delphinidin and petunidin together with pelargonidin and malvidin-based anthocyanins, unusual in tomato. Consistent with PhAN4 features, transcriptomic profiling indicated upregulation of genes correlated to anthocyanin biosynthesis. Interestingly, a transcriptome reprogramming oriented to positive regulation of cell response to biotic, abiotic, and redox stimuli was evidenced. PhAN4 hairy root cultures showed the significant capability to counteract reactive oxygen species (ROS) accumulation and protein misfolding upon high-dose gamma irradiation, which is among the most potent pro-oxidant stress that can be encountered in space. These results may have significance in the engineering of whole tomato plants that can benefit space agriculture.en
dc.description.notesThis work was supported by the ENEA/ASI (Italian Space Agency) BIOExTREME and HORTSPACE Projects (ASI n. 2014-007-R.0, ASI n. 2017-11-H.0).en
dc.description.sponsorshipENEA/ASI (Italian Space Agency) [2014-007-R.0, 2017-11-H.0]en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2022.830931en
dc.identifier.issn1664-462Xen
dc.identifier.other830931en
dc.identifier.pmid35283922en
dc.identifier.urihttp://hdl.handle.net/10919/111321en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMicroTomen
dc.subjecthairy root culturesen
dc.subjectagrospaceen
dc.subjectbiofortificationen
dc.subjectanthocyaninsen
dc.subjectgamma radiationen
dc.titleModifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Spaceen
dc.title.serialFrontiers in Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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