The kinetics of analyte capture on nanoscale sensors
| dc.contributor | Virginia Tech | en |
| dc.contributor.author | Solomon, J. E. | en |
| dc.contributor.author | Paul, Mark R. | en |
| dc.contributor.department | Mechanical Engineering | en |
| dc.date.accessed | 2014-02-05 | en |
| dc.date.accessioned | 2014-02-26T19:10:06Z | en |
| dc.date.available | 2014-02-26T19:10:06Z | en |
| dc.date.issued | 2006-03-01 | en |
| dc.description.abstract | This article presents a number of kinetic analyses related to binding processes relevant to capture of target analyte species in nanoscale cantilever-type devices designed to detect small concentrations of biomolecules. The overall analyte capture efficiency is a crucial measure of the ultimate sensitivity of such devices, and a detailed kinetic analysis tells us how rapidly such measurements may be made. We have analyzed the capture kinetics under a variety of conditions, including the possibility of so-called surface-enhanced ligand capture. One of the modalities studied requires ligand capture through a cross-linking mechanism, and it was found that this mode may provide a robust and sensitive approach to biomolecular detection. For the two modalities studied, we find that detection of specific biomolecules down to concentration levels of 1 nM or less appear to be quite feasible for the device configurations studied. | en |
| dc.description.sponsorship | Caltech BioNems project | en |
| dc.description.sponsorship | Defense Advanced Research Projects Agency Simbiosys F49620-02-1-0085 | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Solomon, JE; Paul, MR. "The kinetics of analyte capture on nanoscale sensors," Biophysical Journal 90(5), 1842-1852 (2006); doi: 10.1529/biophysj.105.067835 | en |
| dc.identifier.doi | https://doi.org/10.1529/biophysj.105.067835 | en |
| dc.identifier.issn | 0006-3495 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/25779 | en |
| dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006349506723712 | en |
| dc.language.iso | en_US | en |
| dc.publisher | CELL PRESS | en |
| dc.rights | In Copyright | en |
| dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
| dc.subject | Coupled chemical-reactions | en |
| dc.subject | Dynamic force microscopy | en |
| dc.subject | Receptors | en |
| dc.subject | Cross-linking | en |
| dc.subject | Cell-surface | en |
| dc.subject | Functional-groups | en |
| dc.subject | Diffusion | en |
| dc.title | The kinetics of analyte capture on nanoscale sensors | en |
| dc.title.serial | Biophysical Journal | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |
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