Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.
| dc.contributor.author | Muñoz Egea, Mari Carmen | en |
| dc.contributor.author | Ji, Pan | en |
| dc.contributor.author | Pruden, Amy | en |
| dc.contributor.author | Falkinham, Joseph O. III | en |
| dc.contributor.department | Civil and Environmental Engineering | en |
| dc.contributor.department | Biological Sciences | en |
| dc.date.accessioned | 2017-09-20T18:35:33Z | en |
| dc.date.available | 2017-09-20T18:35:33Z | en |
| dc.date.issued | 2017-09-14 | en |
| dc.date.updated | 2017-09-20T18:35:33Z | en |
| dc.description.abstract | Both <i>Mycobacterium</i> spp. and <i>Methylobacterium</i> spp. are opportunistic premise plumbing pathogens that are found on pipe surfaces in households. However, examination of data published in prior microbiological surveys indicates that <i>Methylobacterium</i> spp. and <i>Mycobacterium</i> spp. tend not to coexist in the same household plumbing biofilms. That evidence led us to test the hypothesis that <i>Methylobacterium</i> spp. in biofilms could inhibit the adherence of <i>Mycobacterium avium</i>. Measurements of adherence of <i>M. avium</i> cells to stainless steel coupons using both culture and PCR-based methods showed that the presence of <i>Methylobacterium</i> spp. biofilms substantially reduced <i>M. avium</i> adherence and vice versa. That inhibition of <i>M. avium</i> adherence was not reduced by UV-irradiation, cyanide/azide exposure, or autoclaving of the <i>Methylobacterium</i> spp. biofilms. Further, there was no evidence of the production of anti-mycobacterial compounds by biofilm-grown <i>Methylobacterium</i> spp. cells. The results add to understanding of the role of microbial interactions in biofilms as a driving force in the proliferation or inhibition of opportunistic pathogens in premise plumbing, and provide a potential new avenue by which <i>M. avium</i> exposures may be reduced for at-risk individuals. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Muñoz Egea, M.C.; Ji, P.; Pruden, A.; Falkinham III, J.O. Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp.. Pathogens 2017, 6, 42. | en |
| dc.identifier.doi | https://doi.org/10.3390/pathogens6030042 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/79354 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | Mycobacterium avium | en |
| dc.subject | Methylobacterium | en |
| dc.subject | adherence | en |
| dc.subject | biofilm formation | en |
| dc.title | Inhibition of Adherence of Mycobacterium avium to Plumbing Surface Biofilms of Methylobacterium spp. | en |
| dc.title.serial | Pathogens | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |