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Catheter-based Medical Device Biofilm Ablation Using Histotripsy: A Parameter Study

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dc.contributor.authorMorse, Ryanen
dc.contributor.authorChilders, Christopheren
dc.contributor.authorNowak, Elizabethen
dc.contributor.authorRao, Jayasimhaen
dc.contributor.authorVlaisavljevich, Elien
dc.date.accessioned2023-07-10T16:56:30Zen
dc.date.available2023-07-10T16:56:30Zen
dc.date.issued2023-07-01en
dc.date.updated2023-07-05T13:49:02Zen
dc.description.abstractOBJECTIVE: Biofilm formation in medical catheters is a major source of hospital-acquired infections which can produce increased morbidity and mortality for patients. Histotripsy is a non-invasive, non-thermal focused ultrasound therapy and recently has been found to be effective at removal of biofilm from medical catheters. Previously established histotripsy methods for biofilm removal, however, would require several hours of use to effectively treat a full-length medical catheter. Here, we investigate the potential to increase the speed and efficiency with which biofilms can be ablated from catheters using histotripsy. METHODS: Pseudomonas aeruginosa (PA14) biofilms were cultured in in vitro Tygon catheter mimics and treated with histotripsy using a 1 MHz histotripsy transducer and a variety of histotripsy pulsing rates and scanning methods. The improved parameters identified in these studies were then used to explore the bactericidal effect of histotripsy on planktonic PA14 suspended in a catheter mimic. RESULTS: Histotripsy can be used to remove biofilm and kill bacteria at substantially increased speeds compared with previously established methods. Near-complete biofilm removal was achieved at treatment speeds up to 1 cm/s, while a 4.241 log reduction in planktonic bacteria was achieved with 2.4 cm/min treatment. CONCLUSION: These results represent a 500-fold increase in biofilm removal speeds and a 6.2-fold increase in bacterial killing speeds compared with previously published methods. These findings indicate that histotripsy shows promise for the treatment of catheter-associated biofilms and planktonic bacteria in a clinically relevant time frame.en
dc.description.versionAccepted versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.ultrasmedbio.2023.06.010en
dc.identifier.eissn1879-291Xen
dc.identifier.issn0301-5629en
dc.identifier.orcidRao, Jayasimha [0000-0002-0133-2862]en
dc.identifier.otherS0301-5629(23)00203-X (PII)en
dc.identifier.urihttp://hdl.handle.net/10919/115700en
dc.language.isoenen
dc.publisherElsevieren
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37394375en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectAblationen
dc.subjectBacteriaen
dc.subjectBiofilmsen
dc.subjectCatheter-associated infectionsen
dc.subjectHistotripsyen
dc.titleCatheter-based Medical Device Biofilm Ablation Using Histotripsy: A Parameter Studyen
dc.title.serialUltrasound in Medicine and Biologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dcterms.dateAccepted2023-06-13en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicineen
pubs.organisational-group/Virginia Tech/Graduate studentsen
pubs.organisational-group/Virginia Tech/Graduate students/Masters studentsen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/VTC School of Medicine - Instr Pgmsen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicine/General IMen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/VTC School of Medicine - Instr Pgms/VTC School of Medicine-Instr Pgmsen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/TEACH Membersen

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