Engineered Cleistogamy in Camelina sativa for Bioconfinement

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dc.contributor.authorHuang, Debaoen
dc.contributor.authorGao, Liweien
dc.contributor.authorMcAdams, Jeremyen
dc.contributor.authorZhao, Fangzhouen
dc.contributor.authorLu, Hongyanen
dc.contributor.authorWu, Yonghuien
dc.contributor.authorMartin, Jeremyen
dc.contributor.authorSherif, Sherifen
dc.contributor.authorSubramanian, Jayasankaren
dc.contributor.authorDuan, Huien
dc.contributor.authorLiu, Wushengen
dc.date.accessioned2023-01-09T13:56:36Zen
dc.date.available2023-01-09T13:56:36Zen
dc.date.issued2022-12-22en
dc.date.updated2023-01-07T00:53:59Zen
dc.description.abstractCamelina sativa is a self-pollinating and facultative outcrossing oilseed crop. Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition, modified protein profiles, improved seed and oil yield, and enhanced drought resistance. The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into non-transgenic camelina and wild relatives. Thus, effective bioconfinement strategies need to be developed to prevent pollen-mediated gene flow (PMGF) from transgenic camelina. In the present study, we overexpressed the cleistogamy (i.e. floral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina. Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy, affected pollen germination rates after anthesis but not during anthesis, and caused a minor silicle abortion only on the main branches. We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field, and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions. Thus, the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina, and could be used for bioconfinement in other dicot species.en
dc.description.versionAccepted versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.urihttp://hdl.handle.net/10919/113096en
dc.language.isoenen
dc.titleEngineered Cleistogamy in Camelina sativa for Bioconfinementen
dc.title.serialHorticulture Researchen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Alson H. Smith, Jr. ARECen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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