Quantitative Studies of Intracellular Trafficking of Two Classes of Resident Golgi Apparatus Proteins

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dc.contributor.authorStarr, Tregei Nicoleen
dc.contributor.committeechairWilliams, Kimberly Forstenen
dc.contributor.committeememberGoldstein, Aaron S.en
dc.contributor.committeememberStorrie, Brianen
dc.contributor.committeememberSum, Amadeu K.en
dc.contributor.committeememberWalker, Richard A.en
dc.contributor.departmentChemical Engineeringen
dc.date.accessioned2014-03-14T20:10:39Zen
dc.date.adate2006-05-04en
dc.date.available2014-03-14T20:10:39Zen
dc.date.issued2006-04-24en
dc.date.rdate2007-05-04en
dc.date.sdate2006-04-24en
dc.description.abstractThe research presented in this dissertation consists of two primary parts. The initial focus centered on understanding the distribution of Golgi resident glycosyltransferases between the ER and Golgi at steady-state. Retrograde trafficking of these Golgi proteins has been demonstrated experimentally mandating the existence of a dynamic equilibrium between the Golgi apparatus and ER. Our published studies also included the development of a quantitative method for analysis of data collected using fluorescent microscopy. The second part of this dissertation presents results pertaining to the quantification of a unique Golgi resident protein that cycles in the late endosome bypass pathway. Using the published method of analysis and techniques developed during the initial project, the anterograde and retrograde transport kinetics of this Golgi protein were determined and used to develop a compartmental model for pH sensitive trafficking in the bypass pathway. The spatial Golgi distribution of the protein during retrograde transport to the Golgi following endosomal exit was also investigated. This research lies at the interface of experimental cell biology and quantitative computational analysis. These experiments combined more traditional experimental biological approaches with more recent computational approaches to understanding cellular mechanisms. Additionally, development of a quantitative method of analysis validated the use of fluorescent microscopy as a quantitative tool for studying intracellular proteins.en
dc.description.degreePh. D.en
dc.identifier.otheretd-04242006-222423en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04242006-222423/en
dc.identifier.urihttp://hdl.handle.net/10919/27217en
dc.publisherVirginia Techen
dc.relation.haspartTitle.pdfen
dc.relation.haspartDissertation.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectfluorescent microscopyen
dc.subjectglycosyltransferasesen
dc.subjectkinetic modelen
dc.subjectGolgien
dc.subjectconfocal microsocpyen
dc.subjectearly endosomesen
dc.subjectendoplasmic reticulumen
dc.subjectGPP130en
dc.titleQuantitative Studies of Intracellular Trafficking of Two Classes of Resident Golgi Apparatus Proteinsen
dc.typeDissertationen
thesis.degree.disciplineChemical Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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