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The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth

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dc.contributor.authorBartholomew, Holly P.en
dc.contributor.authorReynoso, Guadalupeen
dc.contributor.authorThomas, Brandi J.en
dc.contributor.authorMullins, Chase M.en
dc.contributor.authorSmith, Chastynen
dc.contributor.authorGentzel, Irene N.en
dc.contributor.authorGiese, Laura A.en
dc.contributor.authorMackey, Daviden
dc.contributor.authorStevens, Ann M.en
dc.date.accessioned2022-07-28T14:10:44Zen
dc.date.available2022-07-28T14:10:44Zen
dc.date.issued2022-02-14en
dc.description.abstractThe bacterial phytopathogen Pantoea stewartii subsp. stewartii causes leaf blight and Stewart's wilt disease in susceptible corn varieties. A previous RNA-Seq study examined P. stewartii gene expression patterns during late-stage infection in the xylem, and a Tn-Seq study using a P. stewartii mutant library revealed genes essential for colonization of the xylem. Based on these findings, strains with in-frame chromosomal deletions in the genes encoding seven transcription factors (NsrR, IscR, Nac, Lrp, DSJ_00125, DSJ_03645, and DSJ_18135) and one hypothetical protein (DSJ_21690) were constructed to further evaluate the role of the encoded gene products during in vitro and in planta growth. Assays for capsule production and motility indicate that Lrp plays a role in regulating these two key physiological outputs in vitro. Single infections of each deletion strain into the xylem of corn seedlings determined that Lrp plays a significant role in P. stewartii virulence. In planta xylem competition assays between co-inoculated deletion and the corresponding complementation or wild-type strains as well as in vitro growth curves determined that Lrp controls functions important for P. stewartii colonization and growth in corn plants, whereas IscR may have a more generalized impact on growth. Defining the role of essential transcription factors, such as Lrp, during in planta growth will enable modeling of key components of the P. stewartii regulatory network utilized during growth in corn plants.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fmicb.2021.806504en
dc.identifier.eissn1664-302Xen
dc.identifier.other806504en
dc.identifier.pmid35237242en
dc.identifier.urihttp://hdl.handle.net/10919/111392en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectcornen
dc.subjectmaizeen
dc.subjectPantoea stewartii subspen
dc.subjectstewartiien
dc.subjectphytopathogenen
dc.subjectStewart's wilten
dc.subjecttranscription factoren
dc.subjectxylemen
dc.titleThe Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growthen
dc.title.serialFrontiers in Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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