Fox Hunting in Wild Apples: Searching for Novel Genes in Malus Sieversii
| dc.contributor.author | Wisniewski, Michael | en |
| dc.contributor.author | Artlip, Timothy | en |
| dc.contributor.author | Liu, Jia | en |
| dc.contributor.author | Ma, Jing | en |
| dc.contributor.author | Burchard, Erik | en |
| dc.contributor.author | Norelli, John | en |
| dc.contributor.author | Dardick, Christopher | en |
| dc.contributor.department | Biological Sciences | en |
| dc.date.accessioned | 2021-01-04T12:53:21Z | en |
| dc.date.available | 2021-01-04T12:53:21Z | en |
| dc.date.issued | 2020-12-14 | en |
| dc.date.updated | 2020-12-24T15:29:26Z | en |
| dc.description.abstract | <i>Malus sieversii</i> is considered the progenitor of modern apple (<i>Malus pumila</i>) cultivars and to represent a valuable source of genetic diversity. Despite the importance of <i>M. sieversii</i> as a source of disease resistance, stress tolerance, and novel fruit traits, little is known about gene function and diversity in <i>M. sieversii</i>. Notably, a publicly annotated genome sequence for this species is not available. In the current study, the FOX (Full-length cDNA OvereXpressing) gene hunting system was used to construct a library of transgenic lines of <i>Arabidopsis</i> in which each transgenic line overexpresses a full-length gene obtained from a cDNA library of the PI619283 accession of <i>M. sieversii</i>. The cDNA library was constructed from mRNA obtained from bark tissues collected in late fall–early winter, a time at which many abiotic stress-adaptative genes are expressed. Over 4000 apple FOX <i>Arabidopsis</i> lines have been established from the pool of transgenic seeds and cDNA inserts corresponding to various Gene Ontology (GO) categories have been identified. A total of 160 inserts appear to be novel, with no or limited homology to <i>M. pumila</i>, Arabidopsis, or poplar. Over 1300 lines have also been screened for freezing resistance. The constructed library of transgenic lines provides a valuable genetic resource for exploring gene function and diversity in <i>Malus sieversii.</i> Notably, no such library of t-DNA lines currently exists for any <i>Malus</i> species. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Wisniewski, M.; Artlip, T.; Liu, J.; Ma, J.; Burchard, E.; Norelli, J.; Dardick, C. Fox Hunting in Wild Apples: Searching for Novel Genes in Malus Sieversii. Int. J. Mol. Sci. 2020, 21, 9516. | en |
| dc.identifier.doi | https://doi.org/10.3390/ijms21249516 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/101727 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | apple | en |
| dc.subject | forward over expression | en |
| dc.subject | fox | en |
| dc.subject | malus pumila | en |
| dc.subject | malus sieversii | en |
| dc.subject | malus × domestica | en |
| dc.subject | freezing | en |
| dc.title | Fox Hunting in Wild Apples: Searching for Novel Genes in Malus Sieversii | en |
| dc.title.serial | International Journal of Molecular Science | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |