Expression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicides

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dc.contributor.authorJi, Wanen
dc.contributor.committeechairKushad, Mosbah M.en
dc.contributor.committeememberCramer, Carole L.en
dc.contributor.committeememberHatzios, Kriton K.en
dc.contributor.committeememberHess, John L.en
dc.contributor.committeememberOrcutt, David M.en
dc.contributor.departmentPlant Physiologyen
dc.date.accessioned2014-03-14T21:21:20Zen
dc.date.adate2005-10-14en
dc.date.available2014-03-14T21:21:20Zen
dc.date.issued1991-05-05en
dc.date.rdate2005-10-14en
dc.date.sdate2005-10-14en
dc.description.abstractThe activity of the enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, EC 1.1.1.34) is highly expressed in 4-day-old etiolated seedlings of normal ('DeKalb XL72AA'), dwarf (<u>d₅</u>), and albino (<u>lw₃</u>) maize (<u>Zea mays</u> L.). HMGR activity of maize seedlings appeared to be exclusively associated with the microsomal rather than the plastidic fraction of maize cells. Maize tissues with high meristematic activity such as germinating seeds, leaf bases, root tips, and the site of origin of lateral roots contained high levels of HMGR activity. The activity of HMGR extracted from leaf tips of normal, dwarf, and albino maize seedlings was regulated by light. HMGR activity from leaf tips of 4-day-old maize seedlings was inhibited significantly following exposure to strong light (600 μol/m²/s) for more than 10 h. In contrast, HMGR activity from leaf bases and root tips of maize was not inhibited by exposure to strong light. These results suggest that HMGR may play an important role in cell division and that light may regulate HMGR activity indirectly by increasing cell differentiation. Under conditions of strong light pretreatment with the bleaching herbicides clomazone, norflurazon, fluridone and acifluorfen stimulated by 4-to 7-fold the activity of HMGR extracted from 'Dekalb XL72AA' maize seedlings.en
dc.description.degreePh. D.en
dc.format.extentxi, 103 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-10142005-135755en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-10142005-135755/en
dc.identifier.urihttp://hdl.handle.net/10919/39911en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V856_1991.J5.pdfen
dc.relation.isformatofOCLC# 24119140en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V856 1991.J5en
dc.subject.lcshCorn -- Researchen
dc.titleExpression of 3-hydroxy-3-methylglutaryl coenzyme a reductase in maize as influenced by light and bleaching herbicidesen
dc.typeDissertationen
dc.type.dcmitypeTexten
thesis.degree.disciplinePlant Physiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.leveldoctoralen
thesis.degree.namePh. D.en

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