Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?
| dc.contributor.author | Qureshi, Nasib | en |
| dc.contributor.author | Lin, Xiaoqing | en |
| dc.contributor.author | Tao, Shunhui | en |
| dc.contributor.author | Liu, Siqing | en |
| dc.contributor.author | Huang, Haibo | en |
| dc.contributor.author | Nichols, Nancy N. | en |
| dc.date.accessioned | 2023-07-13T17:08:29Z | en |
| dc.date.available | 2023-07-13T17:08:29Z | en |
| dc.date.issued | 2023-06-26 | en |
| dc.date.updated | 2023-07-13T14:07:07Z | en |
| dc.description.abstract | This study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone butanol ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using <i>Clostridium beijerinckii</i> P260 resulted in a productivity and yield of 0.33 g/L·h and 0.43 g/g, respectively. Use of 60 g/L xylose in a batch system resulted in productivity and yield of 0.26 g/L·h, and 0.40 g/g, respectively. In these two experiments, the culture fermented 89.3% glucose and 83.6% of xylose, respectively. When ABE recovery was coupled with fermentation for continuous solvent removal, the culture fermented all the added xylose (60 g/L). This system resulted in a productivity and yield of 0.66 g/L·h and 0.44 g/g, respectively. When the sugar concentration was further increased above 100 g/L, only a small fraction of the sugar was fermented in batch cultures without product removal. However, with simultaneous product removal, all the xylose (150 g/L) was fermented provided the culture was fed with nutrients intermittently. In this system, 66.32 g/L ABE was produced from 150 g/L xylose with a productivity of 0.44 g/L·h and yield of 0.44 g/g. Using the integrated culture system allowed sugar consumption to be increased by 300% (150 g/L). The continuous system using xylose as a feed did not sustain and after 36 days (864 h) of fermentation, it produced only 2–3 g/L ABE. Rather, the culture became acidogenic and produced 4–5 g/L acids (acetic and butyric). This study suggested that xylose be fermented in batch reactors coupled with simultaneous product recovery rather than in continuous reactors. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Qureshi, N.; Lin, X.; Tao, S.; Liu, S.; Huang, H.; Nichols, N.N. Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?Energies 2023, 16, 4945. | en |
| dc.identifier.doi | https://doi.org/10.3390/en16134945 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/115769 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | butanol | en |
| dc.subject | concentrated xylose | en |
| dc.subject | fermentation | en |
| dc.subject | productivity | en |
| dc.subject | Clostridium beijerinckii P260 | en |
| dc.title | Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There? | en |
| dc.title.serial | Energies | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |