Updating microbial genomic sequences: improving accuracy & innovation

Simple item page
dc.contributor.authorTae, Hongseoken
dc.contributor.authorKarunasena, Enushaen
dc.contributor.authorBavarva, Jasmin H.en
dc.contributor.authorGarner, Harold R.en
dc.date.accessioned2014-11-12T16:03:00Zen
dc.date.available2014-11-12T16:03:00Zen
dc.date.issued2014-11-07en
dc.date.updated2014-11-12T16:03:00Zen
dc.description.abstractBackground Many bacterial genome sequences completed using the Sanger method may contain assembly errors due in-part to low sequence coverage driven by cost. Findings To illustrate the need for re-sequencing of pre-nextgen genomes and to validate sequenced genomes, we conducted a series of experiments, using high coverage sequencing data generated by a Illumina Miseq sequencer to sequence genomic DNAs of Bacteroides fragilis NCTC 9343, Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150, Vibrio cholerae O1 biovar El Tor str. N16961, Bacillus halodurans C-125 and Caulobacter crescentus CB15, which had previously been sequenced by the Sanger method during the early 2000’s. Conclusions This study revealed a number of discrepancies between the published assemblies and sequence read alignments for all five bacterial species, suggesting that the continued use of these error-containing genomes and their genetic information may contribute to false conclusions and/or incorrect future discoveries when they are used.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBioData Mining. 2014 Nov 07;7(1):25en
dc.identifier.doihttps://doi.org/10.1186/1756-0381-7-25en
dc.identifier.urihttp://hdl.handle.net/10919/50840en
dc.language.isoenen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderHongseok Tae et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleUpdating microbial genomic sequences: improving accuracy & innovationen
dc.title.serialBioData Miningen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 2 of 2
Name:
1756-0381-7-25.xml
Size:
35.33 KB
Format:
Extensible Markup Language
Download
Thumbnail Image
Name:
1756-0381-7-25.pdf
Size:
135.23 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, BioMed Central and SpringerOpen
Scholarly Works, Fralin Life Sciences Institute