A fast and simple ion-pair high performance liquid chromatography method for analysis of primary bile salts in in vitro digested bean samples

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dc.contributor.authorLin, Tiantianen
dc.contributor.authorO'Keefe, Sean F.en
dc.contributor.authorFernández-Fraguas, Cristinaen
dc.date.accessioned2021-09-02T17:44:46Zen
dc.date.available2021-09-02T17:44:46Zen
dc.date.issued2021-05-15en
dc.description.abstractBile salts (BS) play a key role in cholesterol and lipid metabolism as well as in many other key metabolic pathways. High performance liquid chromatography (HPLC) is the most common technique used to analyze BS in diverse type of samples. However, current HPLC analysis methods used to analyze and quantify single BS in in vitro digested samples showed poor separation of a complex mixture of BS. In this article, we improved a standard method originally used for quantifying individual BS in food samples subjected to in vitro digestion. We also adapted a method previously developed for BS examination in human blood samples to the analysis of these molecules in chyme samples obtained during simulated gastrointestinal digestion. Our method was simple and achieved a fast and successful separation and quantification of four primary BS (sodium salts of taurocholic, glycocholic, taurochenodeoxycholic and glycochenodeoxycholic acids). •A method used to analyze bile salts in human blood samples has been adapted to separate and quantify four primary bile salts in in vitro digested bean samples. •Addition of an ion-pair reagent led to complete separation of glycine and taurine conjugates of chenodeoxycholic and cholic acids within 10 min, and achieved good peak symmetry. •The minimum BS concentration that could be measured was as low as 0.03125mM.en
dc.description.sponsorshipThe authors acknowledge the funding support from the Dry Bean Health Research Program for the Northarvest Bean Growers Association. This project was also funded by the Virginia Agriculture Experiment Station and the Hatch Program of the National Institute of Food and Agriculture (NIFA), USDA.en
dc.description.versionPublished versionen
dc.format.extent8 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1016/j.foodres.2020.109337en
dc.identifier.issn2215-0161en
dc.identifier.urihttp://hdl.handle.net/10919/104905en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherElsevieren
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectBile saltsen
dc.subjectHPLCen
dc.subjectIon-pair reagenten
dc.subjectIn vitro digestionen
dc.subjectChyme samplesen
dc.titleA fast and simple ion-pair high performance liquid chromatography method for analysis of primary bile salts in in vitro digested bean samplesen
dc.title.serialMethodsXen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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