Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells

Simple item page
dc.contributor.authorBickford, Lissett R.en
dc.contributor.authorLangsner, Robert J.en
dc.contributor.authorChang, Josephen
dc.contributor.authorKennedy, Laura C.en
dc.contributor.authorAgollah, Germaine D.en
dc.contributor.authorDrezek, Rebekahen
dc.contributor.departmentMechanical Engineeringen
dc.contributor.departmentSchool of Biomedical Engineering and Sciencesen
dc.date.accessioned2017-09-18T09:48:00Zen
dc.date.available2017-09-18T09:48:00Zen
dc.date.issued2012-10-22en
dc.date.updated2017-09-18T09:47:59Zen
dc.description.abstractTumor margin detection for patients undergoing breast conservation surgery primarily occurs postoperatively. Previously, we demonstrated that gold nanoshells rapidly enhance contrast of HER2 overexpression in ex vivo tissue sections. Our ultimate objective, however, is to discern HER2 overexpressing tissue from normal tissue in whole, nonsectioned, specimens to facilitate rapid diagnoses. Here, we use targeted nanoshells to quickly and effectively visualize HER2 receptor expression in intact ex vivo human breast tissue specimens. Punch biopsies of human breast tissue were analyzed after a brief 5-minute incubation with and without HER2-targeted silica-gold nanoshells using two-photon microscopy and stereomicroscopy. Labeling was subsequently verified using reflectance confocal microscopy, darkfield hyperspectral imaging, and immunohistochemistry to confirm levels of HER2 expression. Our results suggest that anti-HER2 nanoshells used in tandem with a near-infrared reflectance confocal microscope and a standard stereomicroscope may potentially be used to discern HER2-overexpressing cancerous tissue from normal tissue in near real time and offer a rapid supplement to current diagnostic techniques.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLissett R. Bickford, Robert J. Langsner, Joseph Chang, Laura C. Kennedy, Germaine D. Agollah, and Rebekah Drezek, “Rapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshells,” Journal of Oncology, vol. 2012, Article ID 291898, 10 pages, 2012. doi:10.1155/2012/291898en
dc.identifier.doihttps://doi.org/10.1155/2012/291898en
dc.identifier.urihttp://hdl.handle.net/10919/78995en
dc.language.isoenen
dc.publisherHindawien
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderCopyright © 2012 Lissett R. Bickford et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleRapid Stereomicroscopic Imaging of HER2 Overexpression in Ex Vivo Breast Tissue Using Topically Applied Silica-Based Gold Nanoshellsen
dc.title.serialJournal of Oncologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 2 of 2
Name:
JO.2012.291898.xml
Size:
10.38 KB
Format:
Extensible Markup Language
Download
Thumbnail Image
Name:
JO.2012.291898.pdf
Size:
6.01 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, Hindawi Press
Scholarly Works, Mechanical Engineering
Scholarly Works, School of Biomedical Engineering and Sciences