Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection

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dc.contributor.authorProcko, Carlen
dc.contributor.authorLee, Travisen
dc.contributor.authorBorsuk, Alecaen
dc.contributor.authorBargmann, Bastiaan O. R.en
dc.contributor.authorDabi, Tsegayeen
dc.contributor.authorNery, Joseph R.en
dc.contributor.authorEstelle, Marken
dc.contributor.authorBaird, Lisaen
dc.contributor.authorO'Connor, Carolynen
dc.contributor.authorBrodersen, Craigen
dc.contributor.authorEcker, Joseph R.en
dc.contributor.authorChory, Joanneen
dc.date.accessioned2022-12-13T16:03:20Zen
dc.date.available2022-12-13T16:03:20Zen
dc.date.issued2022-08-25en
dc.description.abstractLeaf cell sorting and scRNA-seq approaches are used to transcriptionally profile the palisade mesophyll layer and provide resources for understanding mesophyll biology. Like other complex multicellular organisms, plants are composed of different cell types with specialized shapes and functions. For example, most laminar leaves consist of multiple photosynthetic cell types. These cell types include the palisade mesophyll, which typically forms one or more cell layers on the adaxial side of the leaf. Despite their importance for photosynthesis, we know little about how palisade cells differ at the molecular level from other photosynthetic cell types. To this end, we have used a combination of cell-specific profiling using fluorescence-activated cell sorting and single-cell RNA-sequencing methods to generate a transcriptional blueprint of the palisade mesophyll in Arabidopsis thaliana leaves. We find that despite their unique morphology, palisade cells are otherwise transcriptionally similar to other photosynthetic cell types. Nevertheless, we show that some genes in the phenylpropanoid biosynthesis pathway have both palisade-enriched expression and are light-regulated. Phenylpropanoid gene activity in the palisade was required for production of the ultraviolet (UV)-B protectant sinapoylmalate, which may protect the palisade and/or other leaf cells against damaging UV light. These findings improve our understanding of how different photosynthetic cell types in the leaf can function uniquely to optimize leaf performance, despite their transcriptional similarities.en
dc.description.notesWe thank J. Mertens, C. Fitzpatrick, L Baum, M. Ku, N. Hah, and L. Ouyang for technical assistance. Some analysis was performed using CyVerse, supported by the NSF (DBI-0735191 and DBI-1743442), and the Flow Cytometry and NGS Core Facilities of the Salk Institute, with funding from NIH-NCI CCSG: P30 014195 and Shared Instrumentation Grant S10-OD023689; and NIH-NCI CCSG: P30 014195, the Chapman Foundation and the Helmsley Charitable Trust, respectively.en
dc.description.sponsorshipNSF [DBI-0735191, DBI-1743442]; NIH-NCI CCSG [P30 014195, S10-OD023689]; Chapman Foundation; Helmsley Charitable Trusten
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/plcell/koac167en
dc.identifier.eissn1532-298Xen
dc.identifier.issn1040-4651en
dc.identifier.issue9en
dc.identifier.pmid35666176en
dc.identifier.urihttp://hdl.handle.net/10919/112868en
dc.identifier.volume34en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectenhancer trapen
dc.subjectexpression patternsen
dc.subjectgene-expressionen
dc.subjectguard-cellsen
dc.subjectb radiationen
dc.subjectarabidopsisen
dc.subjectgrowthen
dc.subjectphloemen
dc.subjectferulate-5-hydroxylaseen
dc.subjectphotosynthesisen
dc.titleLeaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protectionen
dc.title.serialPlant Cellen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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