Focused Ultrasound Biofilm Ablation: Investigation of Histotripsy for the Treatment of Catheter-Associated Urinary Tract Infections (CAUTIs)

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dc.contributor.authorChilders, Christopheren
dc.contributor.authorEdsall, Connoren
dc.contributor.authorGannon, Jessicaen
dc.contributor.authorWhittington, Abby R.en
dc.contributor.authorMuelenaer, Andre A.en
dc.contributor.authorRao, Jayasimhaen
dc.contributor.authorVlaisavljevich, Elien
dc.date.accessioned2022-09-23T19:09:59Zen
dc.date.available2022-09-23T19:09:59Zen
dc.date.issued2021-09-01en
dc.date.updated2022-09-23T18:31:40Zen
dc.description.abstractUrinary catheters often become contaminated with biofilms, resulting in catheter-associated urinary tract infections (CAUTIs) that adversely affect patient outcomes. Histotripsy is a non-invasive focused ultrasound therapy previously developed for the non-invasive ablation of cancerous tumors and soft tissues. Histotripsy has also previously shown the ability to treat biofilms on glass slides and surgical meshes. Here, we investigate the potential of histotripsy for the treatment of CAUTIs for the first time in vitro. Clinically relevant catheter materials (Tygon, Silicone, and latex catheter mimics) and commonly used clinical catheters were tested to determine the feasibility of producing luminal histotripsy bubble clouds. A Pseudomonas aeruginosa (strain PA14) biofilm model was developed and tested to produce luminal biofilms in an in vitro Tygon catheter mimic. This model was treated with histotripsy to determine the ability to remove a luminal biofilm. Finally, the bactericidal effects of histotripsy were tested by treating PA14 suspended inside the Tygon catheter mimic. Results showed that histotripsy produced precise luminal cavitation within all tested catheter mimics and clinical catheters. Histotripsy treatment of a PA14 biofilm with histotripsy reduced luminal biofilm OD590 signal down to background levels. Further, the treatment of suspended PA14 in LB showed a 3.45±0.11 log10 reduction in CFU/mL after 6 histotripsy scans across the catheter mimics. Overall, the results of this study demonstrate the potential of histotripsy to provide a new modality for removing bacterial biofilms from catheter-based medical devices and suggest that additional work is warranted to investigate histotripsy for the treatment of CAUTIs and other biomaterial-associated infections.en
dc.description.versionPublished versionen
dc.format.extentPages 2965-2980en
dc.format.extent16 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1109/TUFFC.2021.3077704en
dc.identifier.eissn1525-8955en
dc.identifier.issn0885-3010en
dc.identifier.issue9en
dc.identifier.orcidVlaisavljevich, Eli [0000-0002-4097-6257]en
dc.identifier.orcidWhittington, Abby [0000-0001-7401-7209]en
dc.identifier.orcidRao, Jayasimha [0000-0002-0133-2862]en
dc.identifier.pmid33950839en
dc.identifier.urihttp://hdl.handle.net/10919/111987en
dc.identifier.volume68en
dc.language.isoenen
dc.publisherIEEEen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000690441000016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCathetersen
dc.subjectMIMICsen
dc.subjectMicroorganismsen
dc.subjectTransducersen
dc.subjectPressure measurementen
dc.subjectUltrasonic imagingen
dc.subjectImagingen
dc.subjectAblationen
dc.subjectbacteriaen
dc.subjectbiofilmsen
dc.subjectcatheter-associated urinary tract infections (CAUTIs)en
dc.subjecthistotripsyen
dc.subjecturinary cathetersen
dc.subjectDEVICE-ASSOCIATED INFECTIONSen
dc.subjectINTRINSIC THRESHOLDen
dc.subjectTISSUE STIFFNESSen
dc.subjectCAVITATIONen
dc.subjectFREQUENCYen
dc.subjectMICROTRIPSYen
dc.subjectPREVENTIONen
dc.subjectDIAGNOSISen
dc.subjectPRESSUREen
dc.subjectTHERAPYen
dc.subjectBioengineeringen
dc.subjectUrologic Diseasesen
dc.subjectInfectious Diseasesen
dc.subjectInfectionen
dc.subject.meshHumansen
dc.subject.meshBiofilmsen
dc.subject.meshPseudomonas aeruginosaen
dc.subject.meshUrinary Tract Infectionsen
dc.subject.meshUrinary Cathetersen
dc.titleFocused Ultrasound Biofilm Ablation: Investigation of Histotripsy for the Treatment of Catheter-Associated Urinary Tract Infections (CAUTIs)en
dc.title.serialIEEE Transactions on Ultrasonics Ferroelectrics and Frequency Controlen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Materials Science and Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Biomedical Engineering and Mechanicsen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicineen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/Internal Medicine/General IMen
pubs.organisational-group/Virginia Tech/VT Carilion School of Medicine/TEACH Membersen

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