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Amino Acids Are an Ineffective Fertilizer for Dunaliella spp. Growth

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dc.contributor.authorMurphree, Colin A.en
dc.contributor.authorDums, Jacob T.en
dc.contributor.authorJain, Siddharth K.en
dc.contributor.authorZhao, Chengsongen
dc.contributor.authorYoung, Danielle Y.en
dc.contributor.authorKhoshnoodi, Nicoleen
dc.contributor.authorTikunov, Andreyen
dc.contributor.authorMacdonald, Jeffreyen
dc.contributor.authorPilot, Guillaumeen
dc.contributor.authorSederoff, Heikeen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-04-29T13:01:15Zen
dc.date.available2019-04-29T13:01:15Zen
dc.date.issued2017-05-26en
dc.description.abstractAutotrophic microalgae are a promising bioproducts platform. However, the fundamental requirements these organisms have for nitrogen fertilizer severely limit the impact and scale of their cultivation. As an alternative to inorganic fertilizers, we investigated the possibility of using amino acids from deconstructed biomass as a nitrogen source in the genus Dunaliella. We found that only four amino acids (glutamine, histidine, cysteine, and tryptophan) rescue Dunaliella spp. growth in nitrogen depleted media, and that supplementation of these amino acids altered the metabolic profile of Dunaliella cells. Our investigations revealed that histidine is transported across the cell membrane, and that glutamine and cysteine are not transported. Rather, glutamine, cysteine, and tryptophan are degraded in solution by a set of oxidative chemical reactions, releasing ammonium that in turn supports growth. Utilization of biomass-derived amino acids is therefore not a suitable option unless additional amino acid nitrogen uptake is enabled through genetic modifications of these algae.en
dc.description.notesThis work was supported by a NSF Emerging Frontiers in Research and Innovation (EFRI) grant [Award Abstract #1332341].en
dc.description.sponsorshipNSF Emerging Frontiers in Research and Innovation (EFRI) [1332341]en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fpls.2017.00847en
dc.identifier.eissn1664-462Xen
dc.identifier.other847en
dc.identifier.pmid28603530en
dc.identifier.urihttp://hdl.handle.net/10919/89248en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectDunaliellaen
dc.subjectnitrogen recyclingen
dc.subjectlipidsen
dc.subjectbiofuelen
dc.subjectamino acidsen
dc.subjectSustainabilityen
dc.titleAmino Acids Are an Ineffective Fertilizer for Dunaliella spp. Growthen
dc.title.serialFrontiers In Plant Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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